When it sees that sequence in a double stranded DNA it will bind there and it will cut.

当它在双链DNA中发现这个序列,就会与之结合并切割

耶鲁公开课 - 生物医学工程探索课程节选

It doesn't cut straight across the double stranded DNA but it cuts in this jagged fashion.

它不直接横切双链DNA,而是留下一个锯齿形切口

耶鲁公开课 - 生物医学工程探索课程节选

The first step is to take the DNA fragments that we're interested in and put them into this vector by basically cutting open the double stranded DNA and inserting the gene that we like in the region where we've cut.

第一步是把我们感兴趣的DNA片段,整合到这个载体里,基本的方法是切开质粒这个双链DNA,把我们想要的基因插入切开的区域

耶鲁公开课 - 生物医学工程探索课程节选