Plasmids turn out to be one of the most powerful and simplest examples of a vector, what's called a vector for delivering DNA into a cell.

质粒被证实是最强大,且最简单的载体形式之一,载体负责将DNA转入细胞

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Let's assume that we have this plasmid cloning vector and we have some pieces of DNA that we would like to put into a plasmid that we would like to make copies of.

假设我们已经有了克隆质粒载体,也有了我们想要将之,整合到质粒上的某些DNA片段,同时也是我们所要复制的DNA片段

耶鲁公开课 - 生物医学工程探索课程节选

The first step is to take the DNA fragments that we're interested in and put them into this vector by basically cutting open the double stranded DNA and inserting the gene that we like in the region where we've cut.

第一步是把我们感兴趣的DNA片段,整合到这个载体里,基本的方法是切开质粒这个双链DNA,把我们想要的基因插入切开的区域

耶鲁公开课 - 生物医学工程探索课程节选

You have to be able to get the DNA into the cell, it has to be incorporated into a vector of some sort, and the vector has to be compatible with the species and the cells that you've provided the DNA to.

你必须把整合在,某种特定载体上的DNA导入细胞,载体也必须和,受体物种,及细胞匹配

耶鲁公开课 - 生物医学工程探索课程节选