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Where does the DNA sequence come from?
我们感兴趣的基因从哪里来
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If I told you that a DNA sequence went 5' to 3' you could draw the whole thing referring back to these notes, right?
如果我告诉你一个DNA链从5'到3',是AGTG,你就可以,画出它的整个结构
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A primer is a short RNA sequence or DNA sequence that gets sort of the process of replication jump started, and that's just because of the biological properties of DNA polymerase that that primer's needed.
引物是一段短的RNA或者DNA序列,引物是整个复制的起点,因为DNA聚合酶的,生物特性,引物在复制过程是必不可少的
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Transcription just means making a single stranded RNA copy of a sequence of base pairs in a DNA.
转录的过程就是从一段DNA碱基序列中,复制出一段单链RNA
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When it sees that sequence in a double stranded DNA it will bind there and it will cut.
当它在双链DNA中发现这个序列,就会与之结合并切割
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Now, in your book, there's an example of plasmid where I've given you the exact sequence of nucleotides that makes up the whole double stranded DNA molecule.
现在在你们书里,有个质粒样本,我将组成这个双链DNA分子的,核苷酸序列写在这里了
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If I took this same restriction enzyme and tried to cut the chromosomal DNA of a sickle patient, it wouldn't cut at that point because the wrong sequence is there.
而如果我用同样的限制酶,去切割病人的染色体DNA,那该酶是不会起作用的,因为基因序列不对
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This label is DNA that might be made radioactive or made fluorescent, and it has a base pair sequence that is from some other region of the gene that you're interested in.
这种标签就是具有放射性,或者荧光性的DNA,它有一个取自于,你感兴趣的那段基因上,其他位置的碱基对序列
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Some of them are changes in the - not the sequence of DNA, not the sequence of nucleotides in the DNA but the chemistry of DNA around that the way that it's packed into a nucleus.
某些改变不是发生在DNA序列上,不是在DNA的核苷酸序列上,而是发生在DNA包装进细胞核的,一系列化学变化中
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There are hundreds,thousands of them known now, and each one has a specific character and one aspects of its character is that it only binds and cuts at a particular sequence of DNA.
已知的有成百上千种,每一种都有自己的特性,特性之一是,只结合和切割特定的某一种DNA序列
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In this case the promoter, which is a sequence of DNA that is positioned right in front of the gene of interest, the promoter is the betagalactoglobulin promoter in this case, and betagalactoglobulin is a milk protein.
这里涉及到的启动子,也是一段DNA序列,它必须刚好位于目的基因序列前端,我们用到的是β-乳球蛋白启动子,β-乳球蛋白是乳汁中的一种蛋白
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Well, what if you knew the sequence for the messenger RNA that made insulin and you designed another single stranded DNA or RNA molecule that was the exact opposite, or the exact compliment, I should say, of that strand?
那么,如果你知道了,合成胰岛素的mRNA的序列,并设计一段与其序列完全相反的,DNA或者RNA分子,应该说是完全互补的一段分子
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Every cell in your body has exactly the same DNA; that is if I could stretch out all the DNA and look at the base pair sequence, the sequences of bases along all the DNA in your chromosomes, they'd be identical in all the cells.
身体内每一个细胞都有完全相同的DNA,如果我把整条DNA展开,然后检查其碱基对的顺序,你体内每个细胞中染色体上,DNA的碱基对顺序,都是完全相同的
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The goal of that was to take for a typical human, or for a typical - in the case of the human genome project maybe you're looking at fruit flies, you want to look at all the DNA in a fruit fly, but to look at the sequence of base pairs that makes up human DNA and write them all out; we'll talk about that later.
这个计划的目的是,建立一个有代表性人类的基因顺序,或者说是典型的人类基因组,就人类基因组计划来说,或许你在研究果蝇,你想观察果蝇内的所有DNA,而人类基因组计划是观察组成人类的,DNA的碱基对顺序并且将它们记录下来,我们不久之后会讨论这些内容
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