Here the unique thing was that there's a restriction site inside that is present in normal DNA and not present in sickle DNA.

刚才例子中,特性就是在正常的基因中,有酶切位点,而在患者的基因中却没有

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If you have DNA fragments, so this is DNA that you've cut up into fragments using restriction enzymes for example.

如果你有DNA片段,例如有一段你用限制酶,切割成片段的DNA

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Now another property of restriction enzymes is that they always cut the DNA in the same way.

限制性内切酶的另一个特性是,总是以同样的方式切割DNA

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Most restriction enzyme also recognize symmetric sequences of DNA, GAATTC for example.

大多数限制性内切酶,也能识别DNA的对称序列,例如GAATTC

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If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.

如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端

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Restriction enzymes are just a kind of enzyme, enzymes are protein molecules that make a chemical reaction go faster, and the chemical reaction that restriction enzymes do is cutting DNA.

限制性内切酶是一种酶,而酶是加速化学反应的蛋白质分子,限制性内切酶加速的化学反应,是切割DNA

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If I took this same restriction enzyme and tried to cut the chromosomal DNA of a sickle patient, it wouldn't cut at that point because the wrong sequence is there.

而如果我用同样的限制酶,去切割病人的染色体DNA,那该酶是不会起作用的,因为基因序列不对

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In a normal cell DNA that DNA in the normal cell - this DNA gets cut by the restriction enzyme, so the sickle gene ends up - so that hemoglobin gene ends up in two pieces.

一个正常细胞的DNA,正常细胞里的DNA,会被限制酶所剪切,因此镰状血红蛋白基因--,不对,是正常血红蛋白基因被切成两段

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Well the first step would be to cut open the plasmid with a particular restriction enzyme, and then what if I take that same restriction enzyme and I cut up the DNA that I'm interested in.

第一步是用某种限制性内切酶把质粒切开,然后用同一种限制性内切酶,切出我想要的DNA

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We're going to take a chromosomal DNA, we're going to digest it with this restriction enzyme, we're going to put it in this tube and run it on a gel, and we're going to see what results down here.

我们取一条染色体DNA,然后用限制酶进行分解,接着再放,在凝胶上进行电泳,之后看看结果如何

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Now if I put them in contact with one another, the plasmid that's been opened and fragments of the DNA - special fragments that I've produced with the same restriction enzyme, they'll have the same sticky ends, they will naturally hybridize with one another.

如果我让它们互相接触,已经切开的质粒和DNA片段--,这DNA片段是我已用同种限制性内切酶,处理过的特别片段,这两者会有相同的粘性末端,它们会自然地相互杂交

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