When it cuts it leaves sticky ends or un-base paired single stranded regions on each end of the part its cut and that's just a property of many restriction enzymes; not all, some cut blunt,just right down the middle.

它切割后会留下两个粘性末端,或者未配对的单链部分,这是许多限制性内切酶的特性,但不是全部,有些限制性内切酶是从中间钝切的

耶鲁公开课 - 生物医学工程探索课程节选

Well one way you could do it is by saying 'if I have this one base pair difference then this sequence is going to be cut by a specific restriction enzyme that recognizes the sequence CTGAGGA'.

一种方法是通过考虑,"如果我有这个不同的碱基对,那该序列必然会,被特定的限制酶识别并切割,这个特定的限制酶能识别,CTGAGGA "

耶鲁公开课 - 生物医学工程探索课程节选