For Hamas leaders, this is,in some ways, a bigger victory than persuading Israel to lift its restriction on goods entering the territory.

VOA: standard.2010.06.16

Here the unique thing was that there's a restriction site inside that is present in normal DNA and not present in sickle DNA.

刚才例子中,特性就是在正常的基因中,有酶切位点,而在患者的基因中却没有

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So that's often a big restriction that a lot of dorms will put. But here, here at law school,

这是许多宿舍明令禁止的。但是在法学院，

关于宿舍制度 - SpeakingMax英语口语达人

That restriction enzyme, there is a restriction enzyme that does that and it's MST-3.

确实存在这种限制酶,叫MST-3

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This restriction enzyme was found in a natural source, it was found in a micro-organism called E.coli.

这个限制性内切酶源于自然界,是从名为大肠杆菌的微生物中发现的

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They do that in a very special way in that they - restriction enzymes are able to identify a particular sequence of bases in a gene.

限制性内切酶的工作机制很特别,它能识别基因中的一段特定的碱基序列

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When it cuts it leaves sticky ends or un-base paired single stranded regions on each end of the part its cut and that's just a property of many restriction enzymes; not all, some cut blunt,just right down the middle.

它切割后会留下两个粘性末端,或者未配对的单链部分,这是许多限制性内切酶的特性,但不是全部,有些限制性内切酶是从中间钝切的

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Restriction enzymes are just a kind of enzyme, enzymes are protein molecules that make a chemical reaction go faster, and the chemical reaction that restriction enzymes do is cutting DNA.

限制性内切酶是一种酶,而酶是加速化学反应的蛋白质分子,限制性内切酶加速的化学反应,是切割DNA

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This gives you a biological mechanism for cutting, using restriction enzymes, and then you denature so that it falls apart, and then you renature so that it comes back together.

这是一种切割DNA的生物机制,用限制性内切酶,改变DNA的性质让它打开,然后让它合起来恢复它的性质

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If I took this same restriction enzyme and tried to cut the chromosomal DNA of a sickle patient, it wouldn't cut at that point because the wrong sequence is there.

而如果我用同样的限制酶,去切割病人的染色体DNA,那该酶是不会起作用的,因为基因序列不对

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Well one way you could do it is by saying 'if I have this one base pair difference then this sequence is going to be cut by a specific restriction enzyme that recognizes the sequence CTGAGGA'.

一种方法是通过考虑,"如果我有这个不同的碱基对,那该序列必然会,被特定的限制酶识别并切割,这个特定的限制酶能识别,CTGAGGA "

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In a normal cell DNA that DNA in the normal cell - this DNA gets cut by the restriction enzyme, so the sickle gene ends up - so that hemoglobin gene ends up in two pieces.

一个正常细胞的DNA,正常细胞里的DNA,会被限制酶所剪切,因此镰状血红蛋白基因--,不对,是正常血红蛋白基因被切成两段

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Well the first step would be to cut open the plasmid with a particular restriction enzyme, and then what if I take that same restriction enzyme and I cut up the DNA that I'm interested in.

第一步是用某种限制性内切酶把质粒切开,然后用同一种限制性内切酶,切出我想要的DNA

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We're going to take a chromosomal DNA, we're going to digest it with this restriction enzyme, we're going to put it in this tube and run it on a gel, and we're going to see what results down here.

我们取一条染色体DNA,然后用限制酶进行分解,接着再放,在凝胶上进行电泳,之后看看结果如何

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