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If it was directly transcribed there'd be regions that are important for making insulin and regions that are not.
如果直接转录的话,那被转录的序列中就会既包括了,和生成胰岛素有关的重要区域,也包括了与之无关的区域
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A pancreas--cell in the pancreas, for example, needs to make insulin and so the gene for insulin is transcribed.
举个例子,一个胰腺细胞,要分泌胰岛素,那么编码胰岛素蛋白的基因就会被转录
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I showed you this in this picture here and those levels of control can be at the level of transcription.
我把这些都给你们显示在这张图上,调控可以在转录的水平下进行
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Now the plasmid started to do its thing, which is replicate and the gene gets transcribed.
那么质粒就可以开始进行复制,基因得以转录
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They're sometimes called transcription factors and this is an example of a transcription factor that is itself activated or turned on by the presence of a steroid.
它们被称为转录因子,转录因子的一个例子就是,能够自激活或被激素激活
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These kinds of molecules which activate genes, they're activating the process of transcription.
这种分子能够激活基因,激活了转录过程
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So, its genetic material wouldn't be released and even if it was released, the genetic material is also cross-linked, and so it can't be transcribed and translated or replicated.
不能释放遗传物质,而且即使能释放遗传物质,该物质也是交联的,因此转录和翻译,即复制过程不能进行
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To make insulin, for example, your cells of your pancreas have to be transcribing that gene, it has to be processed, has to be translated into the protein insulin.
举例来说,如果要合成胰岛素,你的胰腺细胞必须先转录那一段基因,然后进行加工,继而翻译成为胰岛素蛋白
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The most basic control is by controlling when transcription happens.
最基本的途径,是调控细胞转录
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The proteins that are produced by genes that are on the viral genome get transcribed and translated in order to make more structural proteins that are needed for assembly of the virus.
由病毒基因组上的基因,通过转录和转译制造出来的蛋白质,以便可以制造出更多的,被病毒组合所需的结构蛋白
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Now you've heard about reverse transcriptase someplace before,right?
你们听说过反转录酶吧
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Why does it contain reverse transcriptase in it?
为什么它里面有反转录酶
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In this case the shape is - there's a cell body in the center here so this is where the nucleus is and where all the transcription, production of proteins take place here.
在这个细胞中,中间部位是细胞体,细胞核位于其中央,转录以及翻译过程均发生于此
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I'll talk about what that means, encoding insulin means in a minute, but there's a gene that tells your body what insulin looks like and that gets transcribed but only in those cells that make insulin.
一会儿,我将会讨论基因编码胰岛素的意义,所有细胞都含有相同的胰岛素基因,它决定胰岛素的结构以及如何被转录,但是只有胰腺细胞才能产生胰岛素
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Anybody heard of reverse transcriptase?
有人听说过反转录酶吗
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When transcription happens and it turns out that there's a whole biology associated with this, including molecules that are floating around inside your cells called transcription factors, and their job-- they are molecules that are about particular genes and what some of the sequences and are able to turn on those genes inside cells, to make them transcribe.
研究证明,当细胞转录时,还有与之相关的一整套生物机制在运作,包括一种在你细胞中飘浮着的,称为转录因子的分子,它们的工作就是,它们知道该转录哪一段基因,该转录哪一段序列,然后把这些细胞中的基因激活,进行转录
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DNA goes to RNA, goes to protein, that's it, and that is the way it happens in simple organisms like bacteria.
NA转录成RNA接着翻译成蛋白质,仅此而已,而这仅是细菌那样的简单有机体中的机制
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It's called cDNA or complimentary DNA to indicate that it's a copy, a complimentary copy of the messenger RNA.
它叫cDNA或者互补DNA,意味着它是一个拷贝,一个信使RNA反转录得出的DNA拷贝
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RNA polymerase is smart, it knows where it needs to go in order to make the copy of RNA that's required.
NA聚合酶很聪明,它知道该由什么位置介入,来转录所需要的RNA
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Those regions that are not are spliced out during RNA processing to form the mRNA transcript that's used to make the protein.
而这些与生成胰岛素无关的区域,在RNA剪接过程中就被剔除,从而生成转录蛋白质的mRNA
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Transcription just means making a single stranded RNA copy of a sequence of base pairs in a DNA.
转录的过程就是从一段DNA碱基序列中,复制出一段单链RNA
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That information gets put into action through a process, a biological process called transcription, where particular regions of DNA are transcribed into RNA.
信息通过某种过程起到作用,这个生物学过程叫转录,在转录过程中,DNA的特定区域转录成RNA
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It requires RNA processing to happen smoothly, so if you can interfere with RNA processing you can stop a gene from being expressed.
转录需要RNA的顺利加工,因此如果你能干扰RNA加工过程,你就能阻止一段基因的表达
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The end result of transcription is not double stranded DNA, it's single stranded RNA where the RNA that's produced is called messenger RNA.
最终的转录产物并非双链的DNA,而是单链的RNA,称为信使RNA
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It's the transcribed version of DNA, and it's the exact complement of a particular region of DNA.
它是DNA的转录版本,与特定的DNA序列严格互补
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The nucleus contains all the DNA, and so the early stages of gene expression, transcription, happened inside the nucleus where all the DNA is.
NA都在细胞核里,所以早期的基因表达,转录,发生在将所有DNA,都包于其中的细胞核中
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When we talk about gene expression than we're talking about this whole sequence of events I just described: transcription, RNA processing, translation to make the protein.
当我们谈到基因表达的时候,我们就是在讨论,一系列我们刚才提到的过程,即转录,RNA加工,翻译成蛋白质
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We talked very superficially about the biological process going from DNA to protein, so the process of transcription, RNA processing, and translation to produce a protein.
我们粗浅地讲了下,从DNA到蛋白质的生物学过程,即转录,RNA加工,以及翻译得到蛋白质的全过程
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In this same way, this process of transcription which is occurring in cells throughout body all the time is made possible by a protein called RNA polymerase.
NA的合成也很类似,身体内每个细胞内,转录过程的进行都是在一种称作,RNA聚合酶的蛋白质催化下进行的
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It's very important in the biological operation of human cells and that step is RNA splicing, or processing of this RNA, single stranded RNA that's produced by transcription.
而这对人类细胞生物运作至关重要,这个步骤就是RNA剪接,或者说是对RNA的加工,对转录出的单链RNA进行加工的步骤
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