It has to have an origin of replication, which we talked about last time, which is compatible with the cells.

所以质粒必须带有复制起点,我们上次讲过,该起点要与受体细胞匹配

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So what you get on the plate is many copies of the small number of plasmids that you've put in.

我们只要放入少量质粒,就能在培养基上得到大量的复制品

耶鲁公开课 - 生物医学工程探索课程节选

Now the plasmid started to do its thing, which is replicate and the gene gets transcribed.

那么质粒就可以开始进行复制,基因得以转录

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Hopefully each one of those cells is containing one or more of the plasmids that you're interested in and those are being copied as well.

如果顺利的话每个细菌里都含有,一个或更多我们所感兴趣的质粒,而它们也正在被复制

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So having a place that the cell knows - where the cell knows how to replicate is important and so plasmids have an origin of replication.

所以得有一个细胞能识别的区域,能使细胞知道,怎样来复制 这很重要,因此质粒上带有一个复制起点

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A plasmid is a highly tuned machine for making copies of itself.

质粒是一个高度调谐的自我复制的机器

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One of the properties that you would like a plasmid to have is you'd like for cells to be able to replicate it, to make more copies of it.

所以你所希望质粒具备的一个属性,就是得能让细胞复制它,制造出更多的拷贝

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Take a plasmid, cut it open, insert a gene that we want into the plasmid, and then put that plasmid in a host cell, and let the host replicate it.

取一个质粒,把它切开,把目标基因放进去,然后把这个质粒植入到宿主细胞,让宿主来进行复制

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Now one of the big advantages of plasmids,I already mentioned, is that you can take this plasmid and a plasmid is one - you can think of it as a highly tuned machine for copying itself.

质粒的一个很大优势,我提到过,是你可以用这个质粒,而质粒是一个--,你可以把质粒想成,一个高度调谐的自我复制的机器

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We're cloning a gene of interest to us by sort of hijacking that biological mechanism by putting our gene on this plasmid and so our gene gets copied many times, as the plasmid gets copied many times.

我们克隆感兴趣的基因的做法,这个生物机制有点像是绑架,我们把基因绑在质粒身上,随着质粒被多次地复制,我们的基因也一起被多次复制

耶鲁公开课 - 生物医学工程探索课程节选

that means that the cells have to be able to replicate the DNA on the plasmids, in order to make many more copies of it.

就是说这些细胞要能够,复制质粒上的DNA,以得到更多的拷贝

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This region here of this particular plasmid is called the ori or origin of replication.

质粒上的这个区域叫,ori,或者叫复制起点

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Let's assume that we have this plasmid cloning vector and we have some pieces of DNA that we would like to put into a plasmid that we would like to make copies of.

假设我们已经有了克隆质粒载体,也有了我们想要将之,整合到质粒上的某些DNA片段,同时也是我们所要复制的DNA片段

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