You first separated your DNA, let the primers bind, then turn on the enzyme, and it makes copies of each one of these.

首先解旋DNA双链,使引物结合,接着激活聚合酶,最终得到各链的复制产物

耶鲁公开课 - 生物医学工程探索课程节选

Remember we talked about how DNA replicates itself and that there are enzymes, DNA polymerase that bind to the double stranded DNA, separate it, denature it locally, and then start the process of replication.

请记住DNA复制必须有酶的参与,DNA聚合酶结合在双链DNA上,打开双链并原位变性,然后才开始复制

耶鲁公开课 - 生物医学工程探索课程节选