We'll talk about cell culture engineering during Week 4, how do you maintain cells in culture, what are the limits of this.

第四周我们会探讨细胞培养工程,人们如何在培养基中维持细胞的存活,这种方法的局限性是什么

耶鲁公开课 - 生物医学工程探索课程节选

You infect them with the virus, and you let the cells, in culture, produce the virus.

再用病毒感染它们,使培养基中的细胞产生病毒

耶鲁公开课 - 生物医学工程探索课程节选

So, you would grow these monkey kidney cells in culture, you would add some virus to the culture broth, the cells would become infected, the virus would go through its life cycle.

我们在培养基中培养这些猴肾细胞,然偶加入一些脊髓灰质炎病毒,这样细胞就会被感染,病毒开始不断繁殖

耶鲁公开课 - 生物医学工程探索课程节选

In Week 3 you'll actually do some cell culture in the laboratory and look at cultured cells and learn how to manipulate, do some manipulations on cells and culture, and so on throughout the weeks.

第三周你们会在实验室里做一些细胞培养,看看培养基中的细胞都长什么样,了解细胞和培养基的有关操作,整个一周就做这些

耶鲁公开课 - 生物医学工程探索课程节选

So cell culture production wasn't an option.

因此把细胞作为病毒培养基行不通

耶鲁公开课 - 生物医学工程探索课程节选

If we grew these cells on a plate loaded with Ampicillin and we could select cells that have Ampicillin resistance, and this process of selection and cell culture is very important and we'll talk about it more next week.

如果我们在含有氨苄青霉素的,培养基上培养细胞,我们可以筛选出,对氨苄青霉素有抗性的细胞,这个筛选和培养细胞的过程是很重要的,我们下星期会讲到这些

耶鲁公开课 - 生物医学工程探索课程节选

By taking cells from the skin, for example, or cells from your blood or cells from the bone marrow and keeping them alive in culture, we've been able to study how human cells work and learn a lot about the functioning of human organism.

举例来说,通过从人的皮肤,骨髓,或者血液中提取细胞,并在培养基中培养,就能研究人类细胞是如何工作的,并在一定程度上了解人类机体的功能

耶鲁公开课 - 生物医学工程探索课程节选