Take a plasmid, cut it open, insert a gene that we want into the plasmid, and then put that plasmid in a host cell, and let the host replicate it.

取一个质粒,把它切开,把目标基因放进去,然后把这个质粒植入到宿主细胞,让宿主来进行复制

耶鲁公开课 - 生物医学工程探索课程节选

We want not only for the cell to be able to synthesize the DNA, you want it to be able to express the protein off the DNA as well.

我们不仅希望细胞能复制DNA,还希望它能表达目标DNA所编码的蛋白

耶鲁公开课 - 生物医学工程探索课程节选