Took it out of the virus completely, cloned it into a plasmid, that plasmid was expressed in a foreign host, in this case it was expressed in yeast cells.

将基因从病毒中完全提取出来,并克隆制成质粒,该质粒在外来宿主中表达,此例中质粒在酵母细胞中表达

耶鲁公开课 - 生物医学工程探索课程节选

I talked about just not using viruses but using plasmid DNA together with lipids and that's another strategy for gene therapy.

这一部分我们不仅讲了病毒的应用,也讲了利用脂质体包裹的质粒DNA,基因治疗还有其它的策略

耶鲁公开课 - 生物医学工程探索课程节选