If this reforms, so the plasmid reforms back to its native state, that resistance will be recovered.

如果它自己合上了,质粒就会回到原本的状态,抗性就会恢复

耶鲁公开课 - 生物医学工程探索课程节选

If your gene goes in, you've interrupted the gene for antibiotic resistance and those new organisms aren't going to be resistant to antibiotic anymore.

如果基因进入了质粒,原本对抗生素有抗性的基因就被打断了,那些新细菌就不再对抗生素有抗性

耶鲁公开课 - 生物医学工程探索课程节选

There's something in Eliot's--the presentation of Eliot's work and indeed in the work itself that is affronting, resistant, impersonal. And the typography, the presentation of the book is part of that.

艾略特的作品中有一点别的意义，作品展现出来的,本身有点冒犯，具有反抗性和个人性，印刷和，外观也展现了那些特征。

耶鲁公开课 - 现代诗歌课程节选

This process of using a biological event like resistance to Ampicillin in order to pick out the cell population that you're interested in is called selection.

这个利用生物特性,如对氨苄青霉素的抗性,来挑出你所感兴趣的细胞的过程叫筛选

耶鲁公开课 - 生物医学工程探索课程节选

If this plate has Ampicillin in it, then the only cells that would be able to grow here are cells that have resistance to Ampicillin.

如果培养基上有氨苄青霉素,那么能在这里生长的,只有那些对氨苄青霉素有抗性的细胞

耶鲁公开课 - 生物医学工程探索课程节选

If a cell has a gene that makes it resistant to Ampicillin, that means that that micro-organism can survive being exposed to this normally deadly chemical without dying.

如果细胞带有一种基因,能够让它对氨苄青霉素产生抗性,即使把它放在,有致死剂量化学物质的环境中,它也能存活下来

耶鲁公开课 - 生物医学工程探索课程节选

If you selected the cells right than the only ones that have that resistance to Ampicillin are the ones that successfully got your plasmid and are using this Ampicillin resistant gene.

如果你选对了细胞,那么对氨苄青霉素有抗性的就是,成功得到了质粒并表达了基因的细胞

耶鲁公开课 - 生物医学工程探索课程节选

One way that you can look for that gene that you want is by making the cut in your plasmid inside of a gene that encodes for some property like resistance to an antibiotic.

一种找到成功重组质粒的方式,是在质粒控制某种特性的编码区域切割,例如,对抗生素的抗性

耶鲁公开课 - 生物医学工程探索课程节选

You could imagine strategies where you have multiple resistance genes on a plasmid, resistance to Ampicillin, to Penicillin, to Erythromycin for example, and you design strategies for separating out which cells are carrying the plasmid that you're interested in.

若质粒上有多种抗性基因,你就有很多筛选方法,例如对氨苄青霉素,青霉素,红霉素的抗性,而且你可以设计方法分离出,含有你感兴趣质粒的细胞

耶鲁公开课 - 生物医学工程探索课程节选

If we grew these cells on a plate loaded with Ampicillin and we could select cells that have Ampicillin resistance, and this process of selection and cell culture is very important and we'll talk about it more next week.

如果我们在含有氨苄青霉素的,培养基上培养细胞,我们可以筛选出,对氨苄青霉素有抗性的细胞,这个筛选和培养细胞的过程是很重要的,我们下星期会讲到这些

耶鲁公开课 - 生物医学工程探索课程节选

So being a micro-organism that got a plasmid that give you resistance to an antibiotic would be a good thing - that gave you resistance to something that naturally killed cells like you in your environment would be a good thing.

假如一个微生物,因含有质粒而具有抗生素抗性,这实在是一桩美事--,在同样的环境下,普通细胞会被杀死,而你们这些具有抗性的细胞却安然无恙,这实在太棒了

耶鲁公开课 - 生物医学工程探索课程节选

So bacteria that get an unloaded plasmid are going to have resistance to antibiotics.

得到无DNA片段质粒的细菌,就会对抗生素有抗性

耶鲁公开课 - 生物医学工程探索课程节选

The property it gives here is called AMPR or resistance to Ampicillin.

这里给出的这种特性叫做AMPR,即对氨苄青霉素的抗性

耶鲁公开课 - 生物医学工程探索课程节选