One way that that's done is by introducing genes into cancer cells, genes that aren't normally expressed in any mammalian cell, but that will cause the cancer cell to die.

已经成功应用的一种方法是,将基因导入癌细胞,而这种基因通常不在哺乳动物细胞中表达,但是可以导致癌细胞的死亡

耶鲁公开课 - 生物医学工程探索课程节选

If you selected the cells right than the only ones that have that resistance to Ampicillin are the ones that successfully got your plasmid and are using this Ampicillin resistant gene.

如果你选对了细胞,那么对氨苄青霉素有抗性的就是,成功得到了质粒并表达了基因的细胞

耶鲁公开课 - 生物医学工程探索课程节选

In this case, if everything goes right, you inject the DNA into the pronucleus, you implant this fertilized egg into a foster mother, the mother gives birth to offspring, it develops and when it develops this foreign DNA is in the offspring.

如此一来,如果一切顺利,DNA成功注射进原核,受精卵成功植入代孕母亲,成功的孕育了后代,后代健健康康的长大,目的基因就成功的整合进了后代的基因组

耶鲁公开课 - 生物医学工程探索课程节选