The sources are: one,the plasmid vector that we've picked, and the second is these genes that for some reason we're interested in.

第一个是我们选择的质粒载体,第二个是我们感兴趣的基因

耶鲁公开课 - 生物医学工程探索课程节选

You could imagine strategies where you have multiple resistance genes on a plasmid, resistance to Ampicillin, to Penicillin, to Erythromycin for example, and you design strategies for separating out which cells are carrying the plasmid that you're interested in.

若质粒上有多种抗性基因,你就有很多筛选方法,例如对氨苄青霉素,青霉素,红霉素的抗性,而且你可以设计方法分离出,含有你感兴趣质粒的细胞

耶鲁公开课 - 生物医学工程探索课程节选

So we had students do research, quantitative research or qualitative research; we had students do literature reviews in a particular area of interest; work proposals for book they want to write; or workshop proposals and those kinds of things.

我们让学生进行研究，量化研究和质化研究;,我们让学生,就感兴趣的领域写文献观后感;,他们计划写的书的进展计划;,或实验计划等等。

哈佛公开课 - 幸福课课程节选

This is where - this is the region of the plasmid where we're going to insert the DNA that we're interested in.

这就是我们将在质粒上,插入我们感兴趣的DNA的位置

耶鲁公开课 - 生物医学工程探索课程节选

If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.

如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端

耶鲁公开课 - 生物医学工程探索课程节选

The answer is it will want to reform with itself, and if I have these in solution then how many reform with itself and how many reform with the molecule I'm interested probably depends on the relative concentrations of both in the solution and what conditions I have it at.

答案是质粒有可能会自己闭合,如果质粒和我感兴趣的DNA片段,都在溶液里那有多少质粒会自己合上,有多少与我感兴趣的DNA重组呢,这大概取决于两者在溶液里的相对浓度,和我让溶液处在什么条件下

耶鲁公开课 - 生物医学工程探索课程节选