The sources are: one,the plasmid vector that we've picked, and the second is these genes that for some reason we're interested in.

第一个是我们选择的质粒载体,第二个是我们感兴趣的基因

耶鲁公开课 - 生物医学工程探索课程节选

The best way to label this DNA is by designing probes or labels that hybridize with specific sequences that you're interested in.

最好的标记方法是,设计出能与你感兴趣的,特定基因序列杂交的,分子探针或标签

耶鲁公开课 - 生物医学工程探索课程节选

You're not fishing through a whole chromosome in order to find what you want, but you're going to a cell that's already enriched in it.

你不是大海捞针地,在一整个染色体中找基因,而是在一个充满了,你感兴趣的基因的细胞里找

耶鲁公开课 - 生物医学工程探索课程节选

This is another way to clone or make many copies of a gene of interest.

这是另一种克隆出许多,你感兴趣的基因的方法

耶鲁公开课 - 生物医学工程探索课程节选

Where does the DNA sequence come from?

我们感兴趣的基因从哪里来

耶鲁公开课 - 生物医学工程探索课程节选

It's really been a powerful tool in molecular biology because I might have a very small copy of a gene that I'm interested in and I can make enough copies that I can start to do something else with it.

这确实是分子生物学的一项利器,因为我可能只有,很少量的感兴趣的基因拷贝,而我可以马上制造出足够数量的复制品,能使我用作其他用途

耶鲁公开课 - 生物医学工程探索课程节选

We're cloning a gene of interest to us by sort of hijacking that biological mechanism by putting our gene on this plasmid and so our gene gets copied many times, as the plasmid gets copied many times.

我们克隆感兴趣的基因的做法,这个生物机制有点像是绑架,我们把基因绑在质粒身上,随着质粒被多次地复制,我们的基因也一起被多次复制

耶鲁公开课 - 生物医学工程探索课程节选

This is a much more efficient way to get DNA for a gene that you're interested for a couple of reasons.

用这个办法能更有效率地获取你,感兴趣的基因的DNA,原因如下

耶鲁公开课 - 生物医学工程探索课程节选

You can use PCR for fingerprinting as well, for looking in some unknown fluid for example, is a gene that I'm interested in there?

也可以用PCR做生物指纹鉴定,比如在一个未知溶液样本中,里面是不是有我感兴趣的那段基因呢

耶鲁公开课 - 生物医学工程探索课程节选

This label is DNA that might be made radioactive or made fluorescent, and it has a base pair sequence that is from some other region of the gene that you're interested in.

这种标签就是具有放射性,或者荧光性的DNA,它有一个取自于,你感兴趣的那段基因上,其他位置的碱基对序列

耶鲁公开课 - 生物医学工程探索课程节选

Plus,if you're looking for insulin, if you're looking for the gene for insulin you're going to cells that are making it already, they have abundant messenger RNA so it's much easier to separate out and identify the gene that you're interested in.

再者,如果你在找胰岛素,如果你在找胰岛素基因,你要到,正在制造胰岛素的细胞里找,里面有丰富的mRNA,所以更容易分离,并识别出你感兴趣的基因

耶鲁公开课 - 生物医学工程探索课程节选