You then denature again, each one of these strands gets separated, primers bind, turn on the polymerase, a new strand is made.

然后你可以再次使产物链变性,使各链解旋,再结合引物,激活聚合酶,再生成新链

耶鲁公开课 - 生物医学工程探索课程节选

Polymerase needs a primer and that turns out to be important.

聚合酶需要引物,这点非常重要

耶鲁公开课 - 生物医学工程探索课程节选

You take advantage of that, you put it in a test tube, together with nucleotides and primers and your DNA.

利用这一点,把它和核苷酸,引物及DNA一起放进试管

耶鲁公开课 - 生物医学工程探索课程节选

You first separated your DNA, let the primers bind, then turn on the enzyme, and it makes copies of each one of these.

首先解旋DNA双链,使引物结合,接着激活聚合酶,最终得到各链的复制产物

耶鲁公开课 - 生物医学工程探索课程节选

You do that together with primers and with nucleotides, because if you're going to synthesize DNA you need the raw material of DNA, you need the individual nucleotides.

同时把引物和核苷酸放进去,因为合成DNA需要原料,你需要单个的核苷酸

耶鲁公开课 - 生物医学工程探索课程节选

When they separate, the primers that you've added automatically bind through the process of hybridization, and then you turn up the temperature to the optimum for Taq polymerase and DNA synthesis starts.

当双链解旋后,加入的引物就会在杂交的步骤中自动,和解旋后的DNA结合,然后你再将温度升高至,Taq酶的最适温度,DNA就开始合成了

耶鲁公开课 - 生物医学工程探索课程节选

A primer is a short RNA sequence or DNA sequence that gets sort of the process of replication jump started, and that's just because of the biological properties of DNA polymerase that that primer's needed.

引物是一段短的RNA或者DNA序列,引物是整个复制的起点,因为DNA聚合酶的,生物特性,引物在复制过程是必不可少的

耶鲁公开课 - 生物医学工程探索课程节选