This example here, the idea is to introduce a gene into tumor cells of a tumor.

这里的这个例子思路是,将一个基因导入肿瘤组织的肿瘤细胞中

耶鲁公开课 - 生物医学工程探索课程节选

Now, all you have to do is introduce that virus into an organism and the virus will do what it naturally does, which is infect some cells.

剩下你要做的就是将之导入某个生物,病毒自己就会做好自己的本分,也就是去感染细胞

耶鲁公开课 - 生物医学工程探索课程节选

This is the slide that I left on last time and the idea here was to use plasmids, which I talked about last Thursday, to introduce genes into animals.

这是我上节课没讲完的一张幻灯片,这里的思路是利用,上周四所讲过的质粒,以将基因导入动物体内

耶鲁公开课 - 生物医学工程探索课程节选

One way that that's done is by introducing genes into cancer cells, genes that aren't normally expressed in any mammalian cell, but that will cause the cancer cell to die.

已经成功应用的一种方法是,将基因导入癌细胞,而这种基因通常不在哺乳动物细胞中表达,但是可以导致癌细胞的死亡

耶鲁公开课 - 生物医学工程探索课程节选

You get the idea here, is to deliver the gene that you're interested in directly to the tissue where it's being used and in this case, the idea is to introduce a gene that's missing.

但首先你要明确,这种思路是用来将,某个目的基因直接导入,它原本就能被利用的组织中的,此例中是导入一种缺失的基因

耶鲁公开课 - 生物医学工程探索课程节选

There are other vectors that are very efficient at getting DNA into cells.

但是存在某些其它载体,能易如反掌地将DNA导入细胞

耶鲁公开课 - 生物医学工程探索课程节选

Viruses, in particular, are very effective at delivering their viral DNA into cells.

特别是病毒,可以高效地将自身的病毒DNA导入细胞

耶鲁公开课 - 生物医学工程探索课程节选

This solves a major problem which I'm going to talk about just at the beginning of class here in a slide or two, is how to get DNA into cells?

这解决了我要讲到的一个主要问题,就是在这节开始的一两张幻灯片中要讲的,怎样将DNA导入细胞的问题

耶鲁公开课 - 生物医学工程探索课程节选