So you made somehow an anti-sense polynucleotide to the insulin gene or some fraction of the insulin gene.

这样你就合成了,胰岛素基因的反义多聚核苷酸链,或者说胰岛素基因的片段

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How we do that will be clear in a few minutes, but this polylinker as is described down here is a site where you can clone in genes.

马上你们就清楚这该怎么做了,这里所说的多克隆位点,就是你能把基因片段插进来的位点

耶鲁公开课 - 生物医学工程探索课程节选