-
Another example of using this technique is to produce therapeutic proteins from cloned DNA and I'm going to describe this one.
另一个运用到此技术的例子就是,从克隆的基因中制造治疗性的蛋白质,我会具体说这个例子
-
An alternate way is to go to the cell that's making the protein that you want.
另一种方法是,找到制造你想要的蛋白质的细胞
-
One can also purify parts of the protein, that is, parts of the virus, that is, "Do I really need to deliver the whole virus?"
人们还可以纯化部分蛋白质,也就是病毒的某些部分来制造疫苗,那么还需要接种完整的病毒吗
-
Well, one way to get proteins, and it was first used in Hepatitis B, is to find patients, find individuals who are already infected with Hepatitis B.
获得蛋白质的方法之一,也是首次用于制造乙型肝炎疫苗的方法,就是寻找患者,寻找到一些已经感染乙肝的患者
-
The proteins that are produced by genes that are on the viral genome get transcribed and translated in order to make more structural proteins that are needed for assembly of the virus.
由病毒基因组上的基因,通过转录和转译制造出来的蛋白质,以便可以制造出更多的,被病毒组合所需的结构蛋白
-
It turns out in the case of Hepatitis B, the way the lifecycle proceeds-- the cells make too much of the protein and not all of it gets assembled into the virus.
结果显示,在乙肝的病案中,病毒生命周期的发展情况,细胞制造了大量的蛋白质,但并不是所有的蛋白质都会组合成病毒
-
Because they've made many copies of their genome and they've made all the proteins that are necessary to - for assembly of a new virus, then this cell can make many new viruses.
因为病毒制造了大量病毒基因组的拷贝,和所有,新病毒组装所需的蛋白质,所以这个细胞就有能力制造大量新的病毒
-
The important concept is that, while every cell in your body has the capability of making all the proteins that are needed throughout your body, not every cell is doing that at any given time.
这里面有一个重要的概念,就是尽管你身体里的细胞都,有能力制造出,全身上下所需的所有蛋白质,但不是所有细胞每时每刻都在这么做
-
The virus then can self-assemble, that is, you've made a lot of genetic material, you've made a lot of the structural pieces, there has to be some way that the virus can reassemble, repackage itself into active forms.
于是,病毒可以进行自组装了,也就是说,已经制造出大量的遗传物质,也制造出了大量蛋白质外壳,那么病毒就能通过某些方式,重新装配或者重新组合成活性状态
-
If it's making the protein you want, it must be producing messenger RNA with that gene on it.
如果它正在制造你想要的蛋白质,它一定也在制造,你要的基因的mRNA
应用推荐