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Who was also one of the word's first programmers. So she was trying to write this program, and it didn't work.
也是世界上最早的几个程序员之一,她正尝试着完成一个,复杂的求反正切的程序。
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Or you could ask the question, I have this restriction enzyme, at what regions on this plasmid will it cut?
你也可以这样问,我的这种限制性内切酶,会从质粒的哪个区域切开呢
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Or should we think the fundamental thing is the car extended through time ? and sliced up to make car stages?
还是说,根本的部分是贯穿时间的物体,切分成不同的阶段？
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The painting is The Birth of Christ by Botticelli in the end of the 15th century.
这幅油画--《维纳斯的诞生》,是画家波特切利在15世纪作的。
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My head gets cut in half down here and then you could see the brain.
我的头被切成了两半,一直切到这儿,这就是你们所就看到大脑示意图了
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My guess is that our sense of Milton's power, however that power is imagined, is intimately related to the way in which Milton himself represents power in the characters of Satan and of God in Paradise Lost.
我猜我们对弥尔顿力量的理解，不管我们如何想象这力量，与弥尔顿在《失乐园》中所写撒旦和上帝两角色对,力量的展方式切密切相关。
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Cutting involves enzymes called restriction endonucleases or restriction enzymes, which I've already mentioned and they have names.
切割用到的酶叫限制性核酸内切酶,或限制性内切酶,我已经提到过了,而且它们都有名字
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For precisely the reasons that are pointed to by the rest of the argument.
这个论证的其余部分,会为我们指出切确的原因
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Here the unique thing was that there's a restriction site inside that is present in normal DNA and not present in sickle DNA.
刚才例子中,特性就是在正常的基因中,有酶切位点,而在患者的基因中却没有
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That's an example of symmetric sequence and it happens that most restriction enzymes also recognize those spaces.
这是一个对称序列的例子,大多数限制性内切酶都能识别这些序列
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This restriction enzyme was found in a natural source, it was found in a micro-organism called E.coli.
这个限制性内切酶源于自然界,是从名为大肠杆菌的微生物中发现的
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In this case, this particular restriction enzyme cuts symmetrically like this, but not at the same point.
在我们这种情况下,这个限制性内切酶总是这样对称地切割,但两条链切割的位点不一样
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They do that in a very special way in that they - restriction enzymes are able to identify a particular sequence of bases in a gene.
限制性内切酶的工作机制很特别,它能识别基因中的一段特定的碱基序列
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I could cut that up into fragments, and I could search in these fragments to try to find the one that has the insulin gene on it.
我把那部分切成碎片,然后从中找到有胰岛素基因的那一段
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What's really prior is the car itself and talking about car stages is a certain convenience a kind of way of chopping up the fundamental thing of the car.
重要的是,汽车本身,谈论阶段只是提供便利,是一个把基本部分,切分开的方法。
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There are whole catalogs that you go to and buy restriction enzymes.
我们已经可以从整本的目录上,挑选购买限制性内切酶
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That cutting is done by special proteins called restriction enzymes.
切割是由一种特别的蛋白质完成的,称为限制性内切酶
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What are the properties of this restriction enzymes?
这种限制性内切酶的特性是什么
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When it cuts it leaves sticky ends or un-base paired single stranded regions on each end of the part its cut and that's just a property of many restriction enzymes; not all, some cut blunt,just right down the middle.
它切割后会留下两个粘性末端,或者未配对的单链部分,这是许多限制性内切酶的特性,但不是全部,有些限制性内切酶是从中间钝切的
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Moral purity of persons can be achieved only by punishment for heinous sins: for example, the punishment of chirate, or cutting off, ; is a divine punishment of being extirpated from the House of Israel; death, alright, that's one way to be rid of moral impurity.
只有通过惩罚这些穷凶极恶的罪过,才能获得道德的纯洁，举个例子，切去身上的某部分，是一种连根拔去罪恶的惩罚方法;,死亡，当然也是摆脱道德不洁的一种方法。
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Restriction enzymes have names, The names all look - they're all italicized and they're capital letters and small letters so that they won't be easy for you to understand, but they are - if you know the nomenclature, easy to understand.
限制性内切酶有自己的名字,名字看起来都很--,都是斜体的,大写小写字母混合,你们不太容易理解,但它们都是--如果你懂命名法,是很容易理解的
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So I would help myself to this language of space-time worm object that extends not only over space but also over time, and distinguish the entire worm from the various slices or stages that either make up the worm or we could slice the worm into.
然后我借助时间虫这个词,这是描写物体,贯穿时间和空间的词语，并区分出整条虫,和不同的部分,这些部分是组成虫，或者虫可以切分成的。
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That messenger RNA that's being used has already gone through the RNA splicing mechanism and so the introns have already been removed.
这个mRNA,已经经过了RNA剪接机制的剪接,切去了内含子
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Now another property of restriction enzymes is that they always cut the DNA in the same way.
限制性内切酶的另一个特性是,总是以同样的方式切割DNA
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It was found in strain R, a particular strain of E.coli, and it was the first one found, There's a nomenclature that's evolved for this.
酶被发现于R菌株,大肠杆菌中一种特别的菌株,又是被发现的第一种内切酶,有一种专门为此制定的命名法
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If I cut up just DNA from the chromosome, what's called genomic DNA, then I'm going to have both exons and introns within the fragments that I create.
如果我切割的恰好是染色体上的,基因组DNA,那我切成的片段上,就会同时有外显子和内含子
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Most restriction enzyme also recognize symmetric sequences of DNA, GAATTC for example.
大多数限制性内切酶,也能识别DNA的对称序列,例如GAATTC
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Restriction enzymes are just a kind of enzyme, enzymes are protein molecules that make a chemical reaction go faster, and the chemical reaction that restriction enzymes do is cutting DNA.
限制性内切酶是一种酶,而酶是加速化学反应的蛋白质分子,限制性内切酶加速的化学反应,是切割DNA
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If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.
如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端
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