Not every plasmid in your test tube is going to have the right gene inserted in the right way.

不是试管里的每个质粒,都会与正确的DNA片段重组

耶鲁公开课 - 生物医学工程探索课程节选

That's a really good question because if I open this up, why wouldn't it just reform with itself, why would it want to have this in here?

这是个很好的问题,如果我把质粒打开,为什么它不会自己合上,为什么它会与DNA片段重组呢

耶鲁公开课 - 生物医学工程探索课程节选

Some are going to reform and some are going to reform with the gene in, and some probably aren't going to reseal at all under the conditions that I've used.

有些质粒会自己合上,有些质粒会与DNA片段重组并合上,还有的在我提供的条件下,也根本合不上

耶鲁公开课 - 生物医学工程探索课程节选

The answer is it will want to reform with itself, and if I have these in solution then how many reform with itself and how many reform with the molecule I'm interested probably depends on the relative concentrations of both in the solution and what conditions I have it at.

答案是质粒有可能会自己闭合,如果质粒和我感兴趣的DNA片段,都在溶液里那有多少质粒会自己合上,有多少与我感兴趣的DNA重组呢,这大概取决于两者在溶液里的相对浓度,和我让溶液处在什么条件下

耶鲁公开课 - 生物医学工程探索课程节选