The recombinant DHFR protein containing his-tag was purified by using TALON metal affinity resin under nondenaturing conditions.
在非变性条件下，用TALON金属亲和层析树脂纯化含组氨酸标记的重组DHFR。
He pinned the name tag on his jacket.
他把姓名牌别在自己的短外套上。
Consider having any child who has a health problem wear a tag around his neck.
可以考虑让有健康问题的孩子在脖子上戴一个标签。
In January Kodak filed for a patent on an edible RFID tag which could be used, for example, to examine the digestive tract or check whether a patent has taken his medication.
今年一月，Kodak公司已对可食用RFID标记的投放使用提交申请，该产品用于消化道检查及检测病人是否进行药物治疗。
This additional encoding of the original tag is transparent to the user because he or she only wants to see and manage his or her particular role-based tags.
原始标记的这个附加编码对用户是透明的，因为他或她只希望查看和管理自己的基于角色的特定标记。
The explorers hired Charbonneau, a French-Canadian trader, as an interpreter and agreed to let his pregnant wife tag along.
这个探险家雇用了这个法裔加拿大人夏博瑙作为他的口译员，而且还让他那怀孕的妻子一道随行。
Each instance of the message tag includes the author and his or her message.
每个消息的实例都包括作者以及其信息。
A refugee walked into a supermarket of downtown, adapted his eyes to the dizzy light indoorsand peered at the price on the tag ofsome shavers.
一位难民走进市中心的超市，尽量让自己的眼睛适应室内炫目的灯光，细看标签以辨认出那些剃须刀的价格。
DiCaprio has partnered with Swiss watchmaker TAG Heuer on a limited-edition Aquaracer 500M timepiece, with sales benefitting two of his favorite causes.
迪卡普里奥羽瑞士钟表制造商TAGHeuer合作，打造限量版潜航500米计时器，所得的收入用于他所钟爱的两大事业中。
His price tag for two 3, 500-foot tracks with 25 to 30 capsules: a cool $1.7 million.
两套3500英尺轨道，配25到30部吊舱，他居然标价170万美元！
Mougin and his team must also prove that such operations could become cost-effective ways for getting water. The $11.5 million price tag for the one-shot operation is far from that, Simard said.
摩根和他的小组还必须证明这种方式对于获取水来说是合算的，而按照目前计划，一次就要花费1150万美元，这可是个大价钱。
And the maverick made a surprise choice of an unknown running mate that, in the final analysis, undercut his ability to tag Obama as inexperienced and called his judgment into question.
这个特立独行的人令人惊讶地选择了一个名不见经传的竞选伙伴，导致在最后的分析中，他将奥巴马说成缺乏经验的力量严重削弱，而他的判断力则被质疑。
His name tag says he is a doctor.
他的名牌上说他是一名医生。
Does the required character have to say his name or wear a name tag?
指定人物必须自己说出自己的名字，或者佩戴姓名牌吗？
Subject to the availability of quotas on that day applicant may also attend an immigration branch office in person to get a quota tag before his her application be processed.
此外，申请人亦可亲身到入境事务处分区办事处领取当日的筹号，以便办理申请手续，惟需视乎当日有否剩余配额。
A mother bought her son a 99 dollars Halloween costume to scare his friends. "Should I take the price tag off?" the boy asked.
为了让儿子吓唬他的朋友，妈妈花了99元给他买了一套万圣节晚上穿的服装。“我把这价格标签拿下来吧？”男孩吗？
From then on, TAG Heuer continually refresh Record at the same time, has maintained his own style.
从那时起，豪雅表在不断刷新纪录的同时，保持了自己的风格。
His idea was to generate a unique identification tag for each species based on a short stretch of DNA.
他的构想就是以一小段DNA为基础，为每一个物种创造出一种独一无二的鉴定标签。
On the tag was the date of his graduation, and the words "PAID IN FULL".
标签上还有他毕业那天的日期，以及“已付全款”字样。
John was at the tag end of his class.
约翰是他班上最后一名。
Gift parasite refers to someone who doesn't contribute anything in preparing the gift but wants to add his/her name to a gift tag in order to claim partial credit for giving the gift.
（礼物寄生族）指准备礼物的时候完全没有参与或付出，却在送礼物的时候想要在名卡上加上自己的名字，以表示这礼物也有自己的一份。
Dad: There isn't a tag on his face. How do you know? ?
他脸上又没标签。你怎么知道的？
But Gharib believes that with the 'favourite' tag hanging around his neck, the Olympic race could be different.
但是佳里布相信，虽然挂着夺冠热门的招牌，但是奥运会的比赛对于他来说依然是和世锦赛完全不同的感觉。
His name tag says he is a doctor.
他的名牌上说他是一位医生。
And not a single young person in the room except my son, who was then a teenager who would tag along and see his father testify.
却没有一个年轻人在场，除了我儿子，是当时唯一的青少年，除了他没人跟着父亲参加听证会。
And not a single young person in the room except my son, who was then a teenager who would tag along and see his father testify.
却没有一个年轻人在场，除了我儿子，是当时唯一的青少年，除了他没人跟着父亲参加听证会。
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