Objective: To clone, sequence and analyze genome segment 1 (S1) of new isolated reovirus strains.
目的：克隆测序新分离呼肠病毒的S1全长基因组片段，并对其进行序列分析。
Reovirus includes 3 serotypes. Homology of proteins encoded by S1 genome segment was less than 26% between serotypes, while that of viral proteins encoded by other 9 segments was all more than 86%.
呼肠病毒共有3个血清型，不同型之间S1片段编码蛋白的同源性小于26%，其它9个片段编码蛋白的同源性都在86%以上。
If a crop developer uses genetic engineering to delete a discrete segment of a plant genome, how much regulation does that require?
如果某种作物研发者利用基因工程手段除去基因组里的某个片段，这种情形需要何种尺度的监管？
Methods: the blood samples were collected from HFRS patients between 2000 ~ 2001, and the m segment genome of hantavirus was amplified by RT-PCR.
方法：采集2000 ~ 2001年肾综合征出血热(HFRS)患者的血液标本，通过逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒M片断基因。
Methods: the blood samples were collected from HFRS patients between 2000 ~ 2001, and the m segment genome of hantavirus was amplified by RT-PCR.
方法：采集2000 ~ 2001年肾综合征出血热(HFRS)患者的血液标本，通过逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒M片断基因。
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