Established upon the embryo stem cell technique and homologous recombination, gene targeting has been widely used in the genome specific manipulation.
基因打靶技术是建立在胚胎干细胞和同源重组技术之上，可对基因组进行定点修饰的实验方法。
However, nuclear reprogramming, which stands in the way of reconstructed embryo stem cell research, is still a bottleneck in the procedure of nuclear transfer.
但是核重编程仍是核移植技术的瓶颈，制约了重构胚胎干细胞的研究。
However, nuclear reprogramming, which stands in the way of reconstructed embryo stem cell research, is still a bottleneck in the procedure of nuclear transfer.
但是核重编程仍是核移植技术的瓶颈，制约了重构胚胎干细胞的研究。
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