Its immunoreactivity was analyzed by Western blot, ELISA and blocking ELISA using MAb and positive serum.
采用单克隆抗体和阳性血清经elisa、阻断elisa、免疫印迹分析重组蛋白的免疫反应性。
A one step dot ELISA using this specific McAb and a 2nd antibody was used for detection of circulating schistosome antigen in the urine of infected rabbits.
用单抗二抗一步法酶联免疫试验检测感染家兔中的日本血吸虫循环抗原。
Objective To establish a method for the measurement of relative affinity of phage antibodies. Methods The relative affinities of 5 phage antibodies were measured by ELISA using thiocyanate elution.
亲和力是反映抗体特性的一个重要指标，也是抗体研究中经常需要测定的参数，在抗体的筛选、鉴定乃至其功能和应用研究等方面都涉及到亲和力的问题。
Design experiments and optimize assay performance for biomarker compounds using ELISA, SPR, and fluorescence detection.
设计实验和用elisa、SPR和荧光检测等方法优化生物标记化合物的检测。
Methods: Specific antibodies were detected with enzyme linked immunosorbent assay (ELISA) using coated lung tissue as antigen and serum of SARS patients as antibodies.
方法：制备SARS患者肺组织包被抗原，采用酶联免疫吸附试验(EL IS A)检测SARS患者血清中特异性抗体。
The rabies virus antigen contents were detected by double antibody sandwich ELISA assay using polyclonal antibody.
采用多克隆双抗体夹心elisa法快速检测狂犬病毒抗原含量。
Babesiosis equi serum epidemiology investigations in yanbian area were using the EMA-1 genetic recombinant protein as diagnosis antigen by the ELISA.
本试验以EMA - 1基因重组蛋白为诊断抗原，用EL IS A方法对延边地区马巴贝斯虫病进行了血清学调查。
Methods ELISA indirect method, we detected lipopolysacchride core types of E. coli using absorbed lipopolysacchride core type monospecific antisera in E.
方法ELISA间接法，用吸收后单一核心型特异性抗血清检测鉴定E 。
Plasma levels of ACTH and corticosterone were measured using radioimmunoassay and ELISA, respectively.
分别用放免及酶联免疫吸附法（ELISA）测定血浆促肾上腺皮质激素（ACTH）及皮质酮含量。
Detecting sera antibody using ELISA and calculating mice spleen t lymphocytes subgroups (CD4 +, CD8 +) by flow cytometry.
用ELISA方法检测小鼠血清抗体效价，流式细胞仪检测脾t淋巴细胞亚类cd 4 +、CD 8 +数量。
Using sandwich ELISA method, the serum levels of IL-6 of 41 patients in surgical wards were determined before and after operations.
作者应用双抗体夹心ELISA法检测了41例普外科各类手术患者手术前后的IL-6水平变化；
By using the invention, a double-antibody sandwich ELISA method can be conveniently utilized to detect the Eperythrozoon suis.
在本发明的基础上，可方便地应用双抗夹心elisa法检测猪附红细胞体。
Conclusion a double antigen sandwich ELISA have been established using recombinant antigens expressed in E. coli., and can be used to detect anti HEV in different kinds of animals.
结论利用大肠杆菌表达的HEV重组抗原建立了双抗原夹心法elisa，并可同时用于不同动物的抗hev抗体检测。
Following vaccination, the efficacy of vaccine preparations on fish was studied by using nonspecific immune assays and monitoring specific antibody production (ELISA) and artificial challenge tests.
为评价疫苗的免疫效果，从而为鳗弧菌疫苗的推广和应用提供理论根据，还研究了牙鲆和鲈鱼对鳗弧菌疫苗的免疫应答。
This chemiluminescent ELISA, which is offered in low volume microplates, shows increased signal and sensitivity while using a smaller sample size.
此化学发光elisa提供在低量微孔板，提高信号灵敏度，同时使用一个较小的样本量。
An indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) for cypermethrin and cyhalothrin was developed and optimized by using polyclonal antibodies.
利用自制抗体建立并优化了氯氰菊酯和三氟氯氰菊酯的间接竞争ELISA (IC - ELISA)检测方法。
Method Plasma 11-dehydrothromboxane B2(DH-TXB2) and thromboxane B2(TXB2) were detected using ELISA in 38 PIH patients and 20 normal non-pregnant women.
方法采用酶联免疫吸附法（ELISA）检测38例PIH患者血浆11-去氢-血栓烷B2（DH-TXB2），同时测定TXB2，并与正常未孕组比较。
Methods By retrospective analysis and detection of serum antibody in different type paragonimiasis patients using Dot-ELISA and TEST.
方法对斯氏狸殖吸虫病进行临床分析，采用斑点elisa法与IE ST法对不同类型肺吸虫病人血清抗体的检测。
Methods: By using ELISA method, plasma IL8 and IL10 levels were determined in 19 patients with burn injury.
方法：收集19例严重烧伤患者的血浆，利用酶联免疫吸附(elisa)法测定血浆中il 8和IL 10水平。
The results lay the foundation for the further development of ELISA methods by using this monoclonal antibody.
这一结果为用自制单抗进一步开发酶联免疫方法检测奠定了基础。
Methods Serum antibody was detected by using ELISA in 34 patients with angiostrongyliasis, and was followed up for 15 months.
方法采用ELISA检测法，对34例广州管圆线虫病患者进行血清抗体检测，并随访15个月。
Sandwieh ELISA technique using horse anti-fixed rabies virus IgG as coating antibody for detecting rabies antigens in brain samples from sika deer and other animals was established.
以马抗狂犬病固定毒抗体建立的酶联免疫吸附试验夹心法，检测了动物脑组织中的梅花鹿狂犬病病毒抗原。
Methods: the 233 drug users were surveyed using the same questionnaire, HIV antibodies were screened and confirmed using enzyme-linked immunosorbent assay (ELISA) and Western Blot (WB) respectively.
方法：采用同一问卷对强制戒毒所233名吸毒者进行调查，并用酶联免疫吸附试验(ELISA)和确认试验(WB)对吸毒者进行HIV初筛和确认。
Methods: To analyse data of 87 elderly patients by using ELISA method.
方法：采用ELISA方法对87例老年胃癌患者进行回顾性分析。
A double antibody sandwich ELISA for RV antigen was developed using McAb 4c2 as capture antibody and rabbit PcAb as detection antibody.
以单克隆抗体4c2作为捕捉抗体，兔多克隆抗体作为检测抗体，建立了ELISA双抗体夹心法，检测RV抗原。
The results of VEGF detection in the serum of patients with lung cancer were consistent with those using an ELISA Kit.
并利用此体系，检测人肺癌患者血清中的VEGF浓度，其结果与ELISA方法检测出的结果基本一致。
By using avian influenza polyclonal antibody and H9 subtype-specific monoclonal antibody, a H9 subtype specific antigen capture ELISA was developed for detection of H9 subtype avian influenza virus.
采用禽流感病毒多克隆抗体及H9亚型特异性单克隆抗体，研究建立H9亚型特异性抗原捕捉elisa检测方法，用于检测H9亚型禽流感病毒。
The ELISA result using ORF2 protein as antigen showed good accuracy.
以OR F2蛋白为抗原的ELISA检测结果具有较好的准确性。
Using recombinant nucleocapsid protein GST-N of porcine reproductive and respiratory syndrome virus (PRRSV) as antigen, an indirect ELISA for the detection of antibodies against PRRSV was developed.
用亲和层析纯化的猪繁殖与呼吸综合征病毒(PRRSV)重组核衣壳蛋白gst - N作为诊断抗原，建立检测prrs血清抗体的间接ELISA方法。
Using recombinant nucleocapsid protein GST-N of porcine reproductive and respiratory syndrome virus (PRRSV) as antigen, an indirect ELISA for the detection of antibodies against PRRSV was developed.
用亲和层析纯化的猪繁殖与呼吸综合征病毒(PRRSV)重组核衣壳蛋白gst - N作为诊断抗原，建立检测prrs血清抗体的间接ELISA方法。
应用推荐