MethodsThe corresponding designed primers, RT_PCR, gene cloning, DNA sequencing analysis were used.
方法设计相应的引物，用RT _ PCR，基因克隆，DNA序列分析技术。
Ampicilin - resistant transformants were selected and identified by PCR, Enzyme digestion and DNA sequencing analysis.
并对氨苄筛选的重组入外源基因的质粒通过P CR、酶切和测序鉴定。
DNA sequencing analysis proved that the sequence ofthe radiation-inducible promoter was totally consistent with the designing sequence.
合成启动子序列分析与设计序列完全一致；
By PCR-SSCP analysis, 17 PCR products were identified with different mobility of single strand DNA in propositus. 9 suspectable mutations were revealed with DNA sequencing analysis.
SSCP分析发现17个外显子pcr产物单链dna迁移率异常，通过DNA直接测序发现9个外显子可疑突变，但反向测序均未能证实。
Methods immunohistochemistry in 52 cases of nasopharyngeal carcinoma PTEN gene expression, the use of PCR SSCP silver staining, DNA sequencing analysis to detect PTEN gene mutation in exon 5, 8.
方法采用免疫组化检测52例鼻咽癌中pten基因的表达，利用PCRSSCP银染、DNA测序分析等方法检测pten基因第5、8外显子突变。
The popularity of rapid DNA sequencing technology provides large amounts of data for molecular phylogenetics, while the sequence analysis technology is the important tool to gain knowledge from data.
DNA快速测序技术的普及为分子系统学家提供了大量数据，而序列分析技术则是探索数据发现知识的重要工具。
DNA microarray has been applied to DNA sequencing, pharmaceuticals analysis, gene expression and so on.
DNA微集阵列已广泛用于DNA测序、药物分析、基因表达等研究领域。
BACKGROUND: Due to the advanced techniques in sequencing and fragment analysis, DNA sequencers and analyzers produce vast amounts of data within short time.
背景：由于测序和片段分析中的先进技术，DNA测序仪和分析仪在短时间内就产生了大量的数据。
The purified DNA can be used directly in subsequent enzymatic reactions such as labeling, sequencing, cloning, and ligation, as well as for PCR analysis.
纯化的DNA可直接用于后续的应用，如标记、测序、克隆、连接和PCR分析。
Results Enzyme digestion analysis and DNA sequencing showed that TROP2 targeted RNA interference recombinant plasmids were constructed successfully.
结果酶切鉴定和DNA测序分析显示，TROP2靶向RNA干扰重组质粒构建成功。
PCR and single strand conformation polymorphism analysis (SSCP) were combined with DNA sequencing confirmation to screen all 28 exons of SCN5A gene.
采用PCR单链构象多态性技术（SSCP）结合DNA序列测定证实，对病人SCN5A的全部2 8个外显子进行突变检测。
PCR and single strand conformation polymorphism analysis (SSCP) were combined with DNA sequencing confirmation to screen all 28 exons of SCN5A gene.
采用PCR单链构象多态性技术（SSCP）结合DNA序列测定证实，对病人SCN5A的全部2 8个外显子进行突变检测。
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