Objective to explore the optimal condition of direct differentiation into dopaminergic neurons of embryonic stem cells in serum-free and feeder layer cell free medium.
目的寻求无血清、无饲养层细胞存在的情况下，胚胎干细胞向多巴胺能神经元定向诱导分化的最佳条件。
METHODS Xenopus leavis females were injected with HCG, eggs were harvested next morning and dejellied by 2% cysteine, from which the Xenopus leavis egg cell free translating system was prepared.
方法非洲爪蟾注射HCG催卵，2 %半胱氨酸去胶膜，高速离心制备卵提取物，即为非洲爪蟾卵非细胞翻译体系。
A scientist showed that mouse leukemia could be transmitted by cell-free filtrates.
一位科学家发现小鼠白血病可以通过细胞游离滤液传播。
Science, Medicine, and the Future: Cell-free fetal DNA and RNA in maternal blood: implications for safer antenatal testing.
科学，医学，面向未来：母血中无细胞的胎儿DNA和RNA：对安全的产前测试的影响。
The above findings suggest the presence of a crypt repair factor in cell-free filtratespyepared from the intestinal crypt cell suspension of normal mice.
本实验结果提示，在肠腺无细胞滤液中，存在着某种肠腺修复因子，它能促进受照射小鼠肠腺的修复再生。
The establishment of cell-free systems is considered as one of the important advances in the field of cell biology in recent years.
非细胞体系的建立，被认为是近年来细胞生物学领域中的重要进展之一。
Quantitative analysis of fetal cell-free DNA in maternal plasma is of great value in the non-invasive prenatal diagnosis.
提示母体血浆中游离的胎儿dna的定量分析在无创性产前诊断中有重要价值。
Objective:To establish the new technology for detection of fetal cell-free DNA in maternal plasma at early pregnant stage.
目的：建立一种检测早孕妇女血浆中胎儿游离DNA的新方法。
METHODS: We developed an assay based on the targeted analysis of cell-free DNA for the detection of fetal aneuploidies of chromosomes 21, 18, and 13.
方法：我们开发了基于游离DNA定向分析的方法，用于胎儿21号、18号和 13号染色体非整倍体的检测。
Objectives: To establish the technology for extracting cell-free fetal DNA in maternal plasma, comparing detection rate of fetal component marker SRY gene and RASSF1A gene.
工作目的：建立母血浆中胎儿游离dna分离提取的技术，利用SRY基因和RASSF1A基因检测胎儿游离dna，并比较它们的检出率。
Cell-free protein synthesis has some advantages on protein expression and regulation, compared with traditional cell-based expression system.
无细胞蛋白质合成系统相比传统的细胞表达系统有许多优点，包括表达周期短、反应条件容易控制等。
However biochemists have achieved certain steps of the multiplication process in cell-free media.
可是，生物化学家已完成了在无细胞培养基上增殖过程的某些阶段。
Their translation activities were measured in a wheat germ cell-free system.
在麦胚无细胞体外翻译体系中测定了它们的蛋白翻译活力。
Conclusions: Establish effective and steady technology for separating and extracting cell-free fetal DNA in maternal plasma.
结论：建立了有效和稳定地分离提取母血浆中胎儿游离dna的技术。
Methods The cell-free system was prepared by ultracentrifugation of Xenopus eggs and activated by cytochrome C.
方法超速离心制备非洲爪蟾卵无细胞体系，用细胞色素C激活为凋亡体系。
It seemed that the nuclear reconstitution in Xenopus cell-free extracts is independent of not only the resources of exogenous DNA, but also the length of DNA.
实验结果表明，非细胞体系中诱导的核重建不仅与外源dna的种类无关，与外源dna的长度也没有关系。
The improved performance with later gestation is likely attributable to the increased concentration of cell-free fetal DNA within maternal blood as the fetus and placenta develop.
在妊娠期较晚时的测试性能的提高可能与随着胎儿和胎盘的发育母体血液中无细胞胎儿dna浓度的增加有关。
The improved performance with later gestation is likely attributable to the increased concentration of cell-free fetal DNA within maternal blood as the fetus and placenta develop.
在妊娠期较晚时的测试性能的提高可能与随着胎儿和胎盘的发育母体血液中无细胞胎儿dna浓度的增加有关。
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