本发明提供的重组菌可用在生产饲料中。
重组菌通过口服和鼻饲免疫小鼠后，可以诱生CS6血清IgG抗体；
Antibodies against CS6 and LPS of Shigella can be detected in sera of mice immunized with recombinant bacteria either orogastrically (o. g. ) or intranasally (i. n. );
通过与小鼠巨噬细胞株raw264.7共孵育检测重组菌的靶向性。
The targeting property of the recombinant was detected by co-incubating the strains with murine macrophage RAW264.7.
优化培养条件后，此重组菌的发酵酶活单位明显高于野生菌，最高达野生菌的3倍。
After culture optimization, the enzyme production of the recombinant strain was 3 times higher than that of the wild strain.
在诱导表达优化条件下，该重组菌可产生表达量占细菌总蛋白量30%以上的融合蛋白。
Under the optimized expression condition, the recombinant expressed product is account for over 30% of overall bacterium protein;
对重组菌中重组质粒的稳定性进行研究，结果表明该质粒在宿主菌中具有良好的分离稳定性，而结构稳定性较差。
The plasmid stability studies showed that recombinant plasmid has excellent segregational stability but the structural stability was not good in the host cell.
细胞试验表明重组菌对细胞的黏附力和相对侵袭力均有不同程度的降低，而相对侵袭力与野生型菌株具有显著性差异（P<0.05）；
The recombinant strain also had lower adhesiveness(P>0.05)and significantly lower relative invasiveness to the HeLa cells than its wild type strain(P<0.05).
重组菌经培养，利用荧光显微镜检测重组酵母的荧光强度，然后通过荧光分光光度计检测分选酶与底物相互作用后产生游离的EGFP荧光强度进而确定分选酶的酶活。
After cultivation, recombinant cells were verified with fluorescence microscopy and sortase activity was detected by fluorescence spectrophotometer from variety of free EGFP's fluorescence intensity.
正在应用的或处于研究阶段的构建瘤胃基因工程菌的方式主要有基因缺失技术、基因复制性重组技术和启动子的应用技术。
The main methods used, or under investigation, for production of rumen gene engineering bacteria are:gene deletion technique, gene recombination technique and the application of promotor.
目的建立重组NK4融合基因工程菌的发酵工艺。
Objective To develop a fermentation procedure for recombinant E. coli strain expressing NK4 gene.
目的在大肠埃希菌中表达人神经元正五聚蛋白2 (NPTX2)，并对该重组蛋白进行纯化、鉴定。
Objective to construct the prokaryotic expression vector of human neuronal pentraxin 2 (NPTX2) gene, induce the expression of the recombinant fusion protein in e.
所述表达菌包含有本发明的任何一种重组表达载体或本发明任何一种DNA序列。
The expression fungus contains any recombinant expression vector or any DNA sequence of the invention.
对含人EC-SOD重组质粒的工程菌进行诱导表达形成包涵体。
The strains with recombinant plasmid of hEC-SOD were induced and expressed as inclusion body.
采用ELISA夹心法检测人重组IL - 2在酵母菌中表达产物的免疫学活性。
Sandwich ELISA Method for measurement of the activity of recombinant human IL-2 expressed in yeast was established.
目的构建表达P30蛋白抗原表位的重组质粒及工程菌，获得纯化的P30蛋白抗原表位，用于检测弓形虫特异性抗体。
Objective To construct a recombinant plasmid containing P30 gene of Toxoplasma gondii and obtain recombinant protein Methods P30 gene fragment was amplified from genomic DNA of T.
测定了猪生长激素基因工程菌不同表达效率时细菌总蛋白的含量以及包涵体中重组猪生长激素占包涵体总蛋白的比值。
We determined the content of the total bacterial proteins and the proportion of recombinant porcine growth hormone(rpGH)in inclusion bodies(IBs)in the cultures of genetically engineered rpGH E.
在盆栽条件下比较研究了转基因重组根瘤菌与出发菌和参照菌的结瘤与共生固氮效率。
Plant pot experiments were carried out for the recipient strains and their transconjugants to compare their nodulation ability and symbiotic nitrogen fixation efficiency.
测序表明读码框架正确后，将重组子分别转入表达宿主菌e。
After the reading frame was confirmed correct by sequencing, the recombinant plasmid was transformed into expression host e.
此方法可以用于重组溶葡萄球菌酶中残余宿主菌菌体蛋白含量的测定。
The method is simple, sensitive and accurate, so the trace of bacteria protein in recombinant lysostaphin products can be detected.
将工程菌发酵培养，经诱导、表达获得重组蛋白。
Said engineering bacterium is undergone the processes of fermentation, culture, induction, expression to obtain recombinant protein.
重组酵母菌经冻干能有效地实现在非水相中全细胞催化己酸和乙醇酯化合成己酸乙酯。
We constructed a CALB-displaying yeast whole-cell biocatalyst and applied it to esterification from caproic acid and ethanol.
不同基因的系统发育树的不一致之处显示出在黄芪根瘤菌的进化过程中存在基因的水平传递和基因重组。
The incongruent phylogenies of the genes studied suggest that horizontal gene transfer and recombination shape mesorhizobial populations in the gene center of the host plants.
因此，研究人员尝试各种方法构建重组酵母菌以提高木糖发酵能力。
Hence attempts have been made to construct various recombinant yeasts to improve the ability of xylose fermentation.
该免疫毒素质粒的制备方法包括重组质粒的构建、重组质粒转染的工程菌的制备等步骤。
The preparation process of the immunotoxin plasmid includes the construction of the recombinant plasmid, the preparation of the recombinant transinfected engineering bacterium and other steps.
以P25基因的重组表达产物为包被抗原，建立了检测鸭疫里默氏菌血清抗体的间接ELISA。
An indirect ELISA was developed for detecting RA infection in ducks, based on P25 as the coated antigen.
农抗5102产生菌同源菌株9011和FR008的原生质体融合重组子SR7能产生1种两亲本所没有的抗细菌活性物质。
The fusion recombinant SR 7 of the antibiotic 5102 producing homogenous strains 90 11 and FR 008 produced a new antibacterial substance the parents could not.
农抗5102产生菌同源菌株9011和FR008的原生质体融合重组子SR7能产生1种两亲本所没有的抗细菌活性物质。
The fusion recombinant SR 7 of the antibiotic 5102 producing homogenous strains 90 11 and FR 008 produced a new antibacterial substance the parents could not.
应用推荐