方法采用免疫组化法及凝胶电泳迁移率实验。
Methods Immunohistochemistry and electrophoretic mobility shift assay (EMSA) were used.
方法采用免疫组化法对5 3例胰腺癌患者进行IL2R及T细胞定量研究。
Methods IL 2R and T lymphocytes in 53 cases with pancreas carcinoma were examined quantitatively by immunohistochemical method.
采用免疫组化法检测各组大鼠支气管上皮细胞ICAM -1和HO - 1表达。
The expression of ICAM 1 and ho 1 on broncho epithelial cells were detected through immunohistochemistry method.
肺泡巨噬细胞(AM)表面CD 14表达测定采用免疫组化法，并进行计算机图像分析。
The expression of CD14 on alveolar macrophage (AM) surface was assayed by immunohistochemistry and then analyzed with computer imaging system.
采用免疫组化法和计算机图像分析半定量测定各组大鼠气道降钙素基因相关肽蛋白的含量。
Immunohistochemistry and computer image analysis were used to determine semi-quantitatively the content of CGRP protein in airway of rats.
肝内kc表面清道夫受体(SR)、CD 14表达测定采用免疫组化法，并进行计算机图像分析。
The expression of scavenger receptor (SR) and CD14 on KC surface was assayed by immunohistochemistry and then analyzed with computer imaging system.
方法：以3 2例ACC、7例正常腮腺及3例腺泡细胞癌标本为研究对象，采用免疫组化法对NT -3进行检测。
Methods: NT-3 was examined by immunohistochemical analysis in 32 cases of salivary ACC, 7 normal parotid glands and 3 acinic cell carcinoma tissue samples.
方法：采用免疫组化法检测9例丙型肝炎患者肾、心、胰和肠等肝外组织内hcv抗原，并与肝组织内抗原表达进行对比研究。
Methods: Immunohistochemical assay was used to determine the expression of HCV antigen in the kidneys, heart, pancreas, intestine and liver in 9 patients with viral hepatitis c.
采用免疫组化法检测60例肝癌组织及癌旁肝组织中vegf及MMP - 2的表达，用CD 34标记免疫组化法检测微血管密度(MVD)。
Immunohistochemistry was used to determine the expression of VEGF, MMP-2 in 60 HCC tissues and cancer adjacent tissues, and microvessel density (MVD) was detected with labelled CD34.
方法：采用免疫组化（SP法）和细胞图像分析技术。
Method:By using immunohistochemical method(SP) and image analysis technique.
AB PAS特染法检测哮喘小鼠小支气管杯状细胞的分布；同一解剖部位采用免疫组化染色法检测GMCSF的表达。
The goblet cells in small bronchi were identified with AB-PAS staining, and the expression of GM-CSF in the same airway was assessed with immunohistochemistry staining.
方法通过TUNEL法检测三类乳腺癌中凋亡细胞数量及分布比例的差异，采用S-P免疫组化法检测癌周淋巴细胞中CD57抗原表达强度的差异。
Methods The number of apoptosis cells were identified by TUNEL method and the expression intensity of antigen CD57 in lymph cells nearby the carcinoma was determined by S-P method.
方法采用免疫组化SP法检测23例原发性恶性黑素瘤、17例转移性恶性黑素瘤及20例色素痣中骨桥蛋白的表达。
Methods The expression of osteopontin was measured by immunohistochemistry SP technique in 23 primary cutaneous malignant melanoma, 17 metastatic melanoma and 20 nevus pigmentosus.
方法：采用免疫组化s -P法检测41例不明原因不孕症及35例正常宫内膜组织中CD 44的表达水平及分布。
Methods The level of expression of CD44 in 41 unknown reason infertility and 35 normal endometrium was measured by immunohistochemical method (S-P technique).
方法采用免疫组化之SP_TM法，对20例口腔粘膜白斑，20例口腔鳞状细胞癌和10例正常口腔粘膜进行染色。
Methods By immunohistochSP _TM method, staining 20 cases oral leukoplakia, 20 cases squamous cell carcinoma and 10 cases normal mucosa.
方法采用硫酸镍铵增强显色的免疫组化S P法。
Methods Nickel ammonium sulfate intensified immunohistochemical SP technique was used.
方法采用免疫组化s ABC法。
方法：采用免疫组化方法(S - P法)检测105例原发性大肠癌手术切除组织中的微血管计数(mvc)和VEGF、TSP蛋白表达。
Method the microvessel counts (MVC), the expression of VEGF, TSP protein in 105 primary colorectal carcinoma specimens were evaluated by immunohistochemical staining.
方法：采用免疫组化sP法。
方法：采用一步酶法测定血清中MMP -2的表达量，用免疫组化法检测MMP - 2在实体瘤中的表达情况。
Methods: The level of MMP 2 in serum was detected by a step sandwich enzyme immunoassay technique.
标本制成组织芯片，采用免疫组化SABC法，对芯片标本进行染色。
The tissue chips were analyzed by the SABC immunohistochemical method.
方法采用以人血白蛋白攻击方法制备肝纤维化动物模型，放免法测定血TNF ，免疫组化法观察肝内层粘蛋白改变。
Methods The rat models of hepatic fibrosis were established by human serum albumin(HSA) injection. Serum TNF was detected by RIA method and laminin in the liver was studied by immunohistochemistry.
采用生物活性染料- 放射性核素联合示踪法、病理连续切片及免疫组化染色可提高前哨淋巴结活检的检出率及准确性。
Combined techniques and pathologic ultrastaging may increase the identification rate and decrease the false negative rate in patients with SLNB.
方法：采用大鼠甲醛疼痛模型，用免疫组化法观察脊髓cox 2表达的变化。
Methods: COX-2 immunohistochemistry staining was used in rat formalin pain model.
结果石蜡组织切片免疫组化反应预处理，采用中温水浴抗原修复法，其阳性表达率为5 2 .6% ，脱片率为2 .0 % ；
Results Prerteat organization chip, using the middle temperature water bath antigen repairing method, the positive expressing rate is 52.6% and the unfalling rate is 2.0%;
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
方法：采用免疫组化LSAB法，对55例肺癌患者的癌组织及其169枚淋巴结进行检测。
Methods: The expression of P53 and cytokeratin were determined in 169 LNs of 55 patients with lung cancer by LASB method.
方法采用免疫组化SP法和图像分析技术对8例正常肾上腺组织20例肾上腺嗜铬细胞瘤中npy和VIP进行检测。
Methods NPY and VIP were detected by immunohistochemical technique (SP method) and image analysis in 8 cases of normal adrenal, 20 cases of adrenal Pheochromocytoma.
方法采用免疫组化SP法和图像分析技术对8例正常肾上腺组织20例肾上腺嗜铬细胞瘤中npy和VIP进行检测。
Methods NPY and VIP were detected by immunohistochemical technique (SP method) and image analysis in 8 cases of normal adrenal, 20 cases of adrenal Pheochromocytoma.
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