方法：荧光染料粒兰施于移植坐骨神经逆行荧光标记后，在荧光显微镜下观察视网膜神经节细胞。

Methods: fluorescent dyes, Granlar Blue, was used in the retrograde labelling of the graft and retinal ganglion cells were observed under a fluorescent microscope.

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方法应用细胞培养、疫组织化学、荧光标记、光逆行追踪和酶组织化学等技术。

Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.

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方法应用细胞培养、免疫组织化学、核荧光标记、荧光逆行追踪和酶组织化学等技术。

Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.

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方法应用细胞培养、免疫组织化学、核荧光标记、荧光逆行追踪和酶组织化学等技术。

Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.

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