受精卵在含血清FBS或OCS培养液中的囊胚发育率明显高于在无血清培养基中的囊胚发育率。
Presumptive zygotes were cultured in mSOFaa medium supplemented with OCS or FBS had higher blastocyte yield than that in culture medium with free serum.
方法无血清培养胚鼠皮层神经元；
Methods Cortical neuron in embrayonal rats was cultured in non serum ;
方法以无血清培养基体外原代培养角质形成细胞。
Methods Human keratinocytes were primarily cultured in vitro with serum free medium.
说明KSOM可以用于绵羊胚胎早期发育的无血清培养液。
This paper suggested that KSOM can be used as serum free medium for ovine embryo development in vitro.
方法：采用改良的有血清培养技术进行人体角朊细胞的培养。
Methods: The improved cultivating technique of human keratinocyte with serum was used.
目的对无血清培养基与含血清培养基培养cho细胞进行比较。
Objective To evaluate the growth of CHO cells in serum-free and serum-containing media.
在培养后期换用无血清培养基培养，细胞生长及蛋白表达水平正常。
During the later period of the cultivating process, the applying of serum free medium had no significant effects on the cell growth and protein expressing.
细胞培养液中加入一定浓度硒可有效地减轻无血清培养引起的细胞损伤。
Selemum with given concentrations into cell cultures could effectively reduce the damages caused by serum free culture.
采用小鼠神经母细胞癌细胞无血清培养建立神经细胞老化实验研究模型。
Serum-free culture of mouse neuroblastoma cessl were used for the study of neuronal aging, with microfluorometry of the autofluorescence of lipofuscin as the index of neuronal aging.
结论目前无血清培养基尚不能完全取代含血清培养基用于培养CHO细胞。
Conclusion At present, the serum-containing medium for culture of CHO cells can not be completely replaced by serum-free serum.
C6 (R)技术在于它卓越的安全性，可扩展性和生产力下无血清培养条件。
C6 (r) technology lie in its excellent safety profile, scalability and productivity under serum-free culture conditions.
本实验对内蒙古白绒山羊初级毛囊的分离方法和体外无血清培养条件进行了研究。
This experiment was conduced to study the separation method and the serum-free cultured condition of primary follicle of Inner Mongolia Cashmere Goats.
方法：1。采用无血清培养法培养大鼠海马神经元，经免疫荧光法进行纯度鉴定。
Methods:1. Hippocampal neurons of neonatal SD rats were isolated and cultured in the serum-free culture media.
结果表明分散垂体细胞在无血清培养液中能够存活，并具有合成和分泌激素的功能。
It was demonstrated that pituitary cells could survival and synthesize and release hormones in serum-free medium.
方法从人胚胎海马区分离神经干细胞，采用无血清培养基，进行体外扩增培养、传代。
Methods The serum free culturing technology was used to isolate, culture and pass neural stem cells from embryonic human hippocampus.
结果：显示耳蜗螺旋神经节神经元在体外无血清培养条件下，可以存活并进行正常分化。
Results: The spiral ganglion neurons could survive and had a normal phenotypic differentiation in serum-free medium.
结论无蛋白培养基可完全取代常规的有血清培养基，用于生产高滴度、高纯度单克隆抗体。
Conclusions CD medium is able to replace serum containing medium and very suitable for production of high quality McAb.
方法：应用无血清培养基采用小方瓶培养CHO细胞，观察细胞维持时间、培养过程的形态变化。
Methods:Culture CHO cells in serum-free media and then compare the maintainance time, morphological changes and hemagglutination titers of the cells in culture supernatant.
结果无蛋白培养基中单抗的产量与效价比有血清培养的单抗高2～4倍，而且单抗的纯度显著提高。
Results The results showed that the yield, titer and purity of those McAbs were 2-4 times higher than those produced in serum containing medium.
方法：采用无血清培养的新生大鼠海马神经元和额前叶皮质神经元，观察氦氖激光照射对神经元生长发育的影响。
METHODS: the influence of he ne laser irrigation on the growth and development of neurons in hippocampus and cortex of frontal lobe neurons was observed int he cell culture of serum free medium.
另一个难题是，规章制度阻止品尝实验室培养的肉，因为它是用胎血清培养的，胎血清可能包含对人类有危害的污染物。
The other difficulty is that reulgations prevent the lab-grown meat from being tasted, because it has been grown using fetal serum which could contain contaminants that are dangerous to humans.
目的：采用无血清培养体系，研究CD 3单克隆抗体激活的杀伤(CD3AK)细胞诱导HL6 0细胞凋亡情况。
Objective: to investigate whether CD3McAb activated killer (CD3AK) cells could induce apoptosis in HL 60 cells in serum free culture system.
方法从胚胎大鼠的大脑皮质、海马、纹状体等组织分离神经干细胞，用无血清培养技术在体外进行培养、扩增、传代和诱导分化。
The advantage of serum free and clone culturing technology was performed to isolate, culture, passage and induce neural stem cells from embryonic rat cortex, hippocampus and striatum.
在这个无血清培养基中，SSV -NIH3T3细胞可以很好地生长，并具有分泌血小板生长因子样生物学活性物质和致裸鼠肿瘤的能力。
In this SFM SSV-NIH3T3 cells grow well, they keep the ability of secreting platelet-derived growth factor-like material into culture medium and causing tumor growth in nude mice.
方法：利用无血清培养和细胞克隆培养技术，从胚胎大鼠海马、纹状体、脊髓等区分离神经干细胞，进行体外扩增培养、传代、贴壁分化观察。
Methods: The advantage of serum free and clone culturing technology was performed to isolate, culture and passage neural stem cells from embryonic rat hippocampus, striatum and spinal cord.
目的探讨利用肝损伤早期和淤胆血清培养体系从大鼠骨髓细胞中筛选、诱导分化和扩增肝干细胞亚群，以快速、高效地获取骨髓源性肝干细胞。
More and more reports about the application of liver stem cells in hepatic diseases appeared, especially about bone marrow-derived liver stem cells isolated from bone marrow.
在牛卵母细胞体外成熟培养过程中，本研究在同一种成熟培养液中，分别添加用0.3％PVP和10％FBS，检验PVP代血清培养是否可行。
During the bovine oocyte maturation, this research examined the possibility of serum-free maturation which 10%FBS was replaced with 0.3%PVP.
试验研究了培养液中血清的添加时间和添加浓度及不同培养体系对黄牛孤雌胚胎体外发育潜力的影响。
The effects of fetal bovine serum (FBS) addition time, concentration and different culture systems on the developmental potentiality of bovine parthenogenetic embryos were observed.
试验研究了培养液中血清的添加时间和添加浓度及不同培养体系对黄牛孤雌胚胎体外发育潜力的影响。
The effects of fetal bovine serum (FBS) addition time, concentration and different culture systems on the developmental potentiality of bovine parthenogenetic embryos were observed.
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