目的探讨大鼠精原细胞的分离纯化及双酚a对精原细胞活力和结构的影响。
Objective: to study isolation and purification of rat spermatogonia and effects of bisphenol a on activities and morphology and structure of spermatogonia in vitro.
目的改良现有的小胶质细胞纯化分离培养方法，建立稳定简便的培养模型。
Objective To modify the current cultural method of rat microglia and establish a convenient and stable model of culture method for rat microglia.
采用亲和层析和离子交换层析分离纯化，以细胞增殖实验测定表达蛋白的生物活性。
Affinity chromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
目的优化胰岛细胞分离纯化的方法，提高胰岛产量、纯度和功能。
Objective To improve output, purification and function of islet cells by optimizing isolation and purification methods.
分离纯化后的细胞具有自我更新能力和多分化潜能，有很强的增殖能力。
The separated and purified cells were of self-renewing ability, multi-differentiating potential, and strong proliferative ability.
文章就骨髓源性肝干细胞的分离、培养、纯化、诱导分化、检测及肝内移植的可能机制和应用前景进行综述。
The article covers isolation, culture, differentiation, tests, possible mechanisms of engraftment in the liver and application prospect of bone marrow-derived liver stem cells.
本文研究了波尔山羊耳成纤维细胞的体外培养，包括分离纯化和生长特征，同时还研究了成纤维细胞的冷藏和冷冻保存。
The paper is mainly about the culture of Boer goat ear fibroblasts, including the isolation, purification and growth character, and we also studied the cold storage and cryopreservation.
从细胞萃取液或生物液体中分离酶的方法，基本上就是纯化蛋白质的方法。
The methods employed fOR enzyme isolation from cell extracts OR from biological fluids are essentially those fOR the purification of proteins.
通过分离、纯化细胞核的对照实验证明不同培养条件对烟草细胞悬浮系的生长和周期具有显著影响。
The controlled experiment of isolating and purifying nuclei proves that the culture conditions work on the growth of the tobacco suspension cells obviously.
目的：研究分离纯化的透明质酸寡糖片段(o - HA)对血管内皮细胞增殖的作用。
Objective: To investigate the effect of purified hyaluronan oligosaccharides (o-HA) on endothelial cells (ec) proliferation and wound healing.
目的：建立免疫磁珠法体外分离纯化人牙周膜干细胞的方法。
Objective: To isolate and identify the periodontal ligament stem cells from human molars.
因此，对于研究发育生物学而言，能够分离纯化、培养和有效利用胚胎后亚全能干细胞，无疑具有非常重要的意义。
So the ability to purify, culture, and manipulate these postembryonic stem cells would provide investigators with an invaluable cell source to study cell and organ development.
目前的研究多对肝卵圆细胞进行活化、分离纯化、培养鉴定，再通过各种方法将其诱导分化为胰岛细胞。
The present researches were to active, separate, purify, cultivate and identify the HOCs, then all kinds of methods were used to induce it to differentiate into islet cells.
结论为各种胰岛细胞的实验研究建立了快速分离纯化胰岛细胞的方法，纯化的胰岛细胞功能良好。
Conclusion We have established a simple and rapid rat islet purification method for islet study, and the purified islet cells remain full function.
目的探讨建立人皮肤汗腺细胞体外分离及纯化培养的技术。
Objective To investigate the method of isolation and purification of epithelial cells of human eccrine sweat gland in vitro.
目的建立脱脂棉柱纯化人外周血T淋巴细胞的方法，并与尼龙棉柱分离法进行比较。
Objective To establish a method to purify T lymphocytes from human peripheral blood mononuclear cells (PBMCs) using cotton column and compare its result with a nylon wool cotton column.
方法用RT - PCR方法从能分泌特异性抗人afp单克隆抗体的杂交瘤细胞中分离纯化抗体VH和VL基因。
Methods VH and VL genes of anti-human AFP monoclonal antibody were cloned by RT-PCR from hybridoma.
目的比较免疫磁珠法和密度梯度离心法分离纯化人外周血中性粒细胞的可靠性。
Objective To compare the reliability of immunomagnetic beads and density gradient centrifugation in purification and isolation of neutrophilic granulocytes from human peripheral blood.
方法：将从小鼠肌肉分离、纯化的旋毛虫幼虫，放入玻璃匀浆器匀浆，胰酶消化。台盼蓝和中性红染色检测分离细胞的活性。
Methods: Larvae of Trichinella spiralis isolated from the muscle of a Trichinella - infected mouse were put in a glass homogenizer for homogenizing, digesting with trypsin.
然而，目前正被应用的滋养层细胞分离、培养、纯化方法均存在一定程度的缺陷。
However, the primary cell culture methods which are employed now have many defects.
方法应用改良的准分子激光切削法和酶分层消化法来分离纯化视网膜感光细胞层。
MethodsModified excimer laser surgery, regular excimer laser surgery, and enzyme gradation digestion were used to isolate retinal photoreceptors.
在DMEM培养基中进行了原代及传代培养，对其中的成纤维细胞进行分离纯化。
The isolated cells were cultured in DMEM medium and PDL fibroblasts were purified in subculture.
目的建立一种快速分离、纯化肿瘤组织癌细胞并提取微量rna的方法。
Objective To establish a rapid method for isolating and purifying cancer cells from tumor tissue and for RNA extraction from tiny amount of the cells thus obtained.
目的介绍一种快速原代培养、分离、纯化视神经星形胶质细胞的方法。
Objective To introduce a method for condition primary culture of astrocyte of optic nerve.
方法利用盐酸利多卡因注射液联合震荡培养法分离纯化胎大鼠脑内小胶质细胞，免疫荧光技术及流式细胞技术鉴定小胶质细胞的纯度。
Method: Fetal rat microglia in brain was isolated and purified by lidocaine hydrochloride with shock culturing. The purification of microglia was detected by immunofluorescence and flow cytometry.
方法利用盐酸利多卡因注射液联合震荡培养法分离纯化胎大鼠脑内小胶质细胞，免疫荧光技术及流式细胞技术鉴定小胶质细胞的纯度。
Method: Fetal rat microglia in brain was isolated and purified by lidocaine hydrochloride with shock culturing. The purification of microglia was detected by immunofluorescence and flow cytometry.
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