此外，讨论了用组织培养方法生产左旋多巴的可能性和固定化细胞培养等问题。
The possibility of the production of L-dopa by tissue culture of Mucuna and the problems in immobilized cell culture were discussed.
采用组织培养和单层细胞培养两种方法对SD大鼠的胰腺进行培养。
The tissue culture and the single layer cell culture techniques for SD rat pancreatic islets were studied.
通过愈伤组织培养、悬浮细胞培养和原生质体培养方法对草坪草的一些种已建立较为完善的植株再生体系。
The plant regeneration systems of some turfgrass species have been established perfectly through callus culture, cell suspension culture, and protoplast suspension culture.
在植物体细胞组织培养中，蔗糖一直作为标准碳源，然而其他糖类物质如：葡萄糖、麦芽糖和山梨醇对植物体细胞培养也产生了一定的影响。
In somatic culture, sucrose has been a standard carbon source, however other kinds of sugar such as glucose, maltose and sorbitol have a certain influence on in vitro tissue culture.
应用PCR方法检测组织细胞培养物中的病毒增殖和增殖滴度，测定了PCV2病毒收获时间，试验表明PCV2病毒在PK15组织培养可连续传代，增殖病毒。
PCR method was applied to detect the proliferation condition and titer of PCV2 and to determine the harvest time of PCV2. The results showed that PCV2 could proliferate and passage on PK15 cells.
通过愈伤组织培养、悬浮细胞培养、原生质体培养等手段，对早熟禾属、黑麦草属、结缕草属和剪股颖属的一些物种已建立了较为完善的植株再生体系。
Plant regeneration system of the species of genus Lolium, Poa, Zoysia, Agrostis and Festuca were established by means of solid medium culture, suspension culture, and protoplast suspension culture.
通过愈伤组织培养、悬浮细胞培养、原生质体培养等手段，对早熟禾属、黑麦草属、结缕草属和剪股颖属的一些物种已建立了较为完善的植株再生体系。
Plant regeneration system of the species of genus Lolium, Poa, Zoysia, Agrostis and Festuca were established by means of solid medium culture, suspension culture, and protoplast suspension culture.
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