结论：滤纸条取液技术取样作沙眼衣原体的多聚酶链反应检测效果好。
The positive rates between different types of tubal pathological changes are not obviously different. Conclusions: The filter paper technique is an effective method in detecting CT-DNA by PCR.
方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
方法应用多聚酶链反应技术及限制性片段长度多态现象对46例食管癌APC和MCC基因的LOH进行了分析。
Methods LOH at APC and MCC genetic loci in 46 specimens resected from esophageal neoplasm was studied with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).
一种定量实时多聚酶链反应能够快速准确的诊断巨细胞病毒，改善免疫抑制患者的治疗。
A quantitative real-time polymerase chain reaction test provides rapid and accurate diagnosis of cytomegalovirus and improved management of immunocompromised patients.
采用半定量逆转录多聚酶链反应(RT - PCR)检测局部内皮素系统ET - 1、ETAR、ETBR及ECE的基因表达。
The gene expression of ET-1, ETAR, ETBR and ECE was evaluated by semi-quantitative reverse transcription polymerase chain response (RT-PCR).
方法应用逆转录酶-多聚酶链反应(RT - PCR)方法，检测了83例食管癌和贲门癌组织及28例患者45枚淋巴结中MRP基因的表达，并与相应癌旁组织进行对照分析。
Methods RT-PCR was applied to study expression of MRP gene in tumor tissues from 83 cases of esophageal and cardiac carcinoma, and 45 lymph nodes from 28 patients.
多聚酶链反应(PCR)具有灵敏度高，特异性强，快速高效的特点，在病原微生物检测领域有广阔的前景。
Polymerase chain reaction (PCR) has many advantages, such as high sensitivity, strong specificity and high-speed. There are vast vistas in the examination of pathogenic microorganism.
所用的DNA方法学和所处的妊娠期对检测精度有着最大的影响，实时定量多聚酶链反应(RTQ -PCR)的检测精度超过常规的PCR。
DNA methodology and gestational age had the largest effects on test performance, with real-time quantitative polymerase chain reaction (RTQ-PCR) outperforming conventional PCR.
我们将人脑动脉瘤和动静脉血管畸形中的内皮细胞分离出来，并用内皮标志物结合多聚酶链反应和免疫组化等方法确认其内皮来源性。
We isolated ECs from human AVM and aneurysm and then confirmed their EC origin by polymerase chain reaction and immunocytochemistry with endothelial markers.
采用多聚酶链反应-限制性片段长度多态性法(PCR - RFLP)分析MGP和ALAD基因的多态性。
The polymorphisms of MGP gene and ALAD gene were analyzed by the methods of PCR-RFLP.
方法：运用多聚酶链反应-限制性内切酶片段长度多态性技术(PCR -RFLP)检测MTHFR的677位点多态性。
Methods: PCR-RFLP technique was used for detecting the A677V polymorphism site of MTHFR gene.
方法：运用多聚酶链反应-限制性内切酶片段长度多态性技术(PCR -RFLP)检测MTHFR的677位点多态性。
Methods: PCR-RFLP technique was used for detecting the A677V polymorphism site of MTHFR gene.
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