目的观察海洋假单胞菌碱性蛋白酶（MPAP）的溶栓和抑制血栓形成的作用，研究其对纤溶、凝血和血小板聚集功能的影响。
ObjectiverTo observe the thrombolysis and anti-thrombosis effects of MPAP; To study the actions of MPAP on blood coagulation, platelet aggregation and fibrinolytic activity.
超声波的引入，不仅大大提高了低温加碱的溶胞效果，而且对溶胞过程温度的要求有所降低。
The introduction of ultrasonic wave not only greatly improved the effect of lysis, but also decreased the requirements for the temperature during the lysis.
在某些方法中，将所述溶胞产物在一定温度下加热一定时间，所述温度和时间足以使所述溶胞产物中存在的蛋白质变性并使其聚集和沉淀。
In certain methods, the lysate is heated for a time and at a temperature sufficient to denature proteins present in the lysate and cause their aggregation and precipitation.
目的用亲和纯化法纯化嗜水气单胞菌气溶素，从单因子水平分析嗜水气单胞菌的溶血特性。
Methods:The proteins which interacted with gankyrin were screened and analyzed by affinity purification technology and mass spectrometry.
进一步提供了可选择的额外的纯化步骤，包括溶胞产物过滤、阴离子交换层析、三联体亲和层析和疏水性相互作用层析。
It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatography, and hydrophobic interaction chromatography.
据报道，纳豆激酶有和胞浆素一样的可直接分解纤维蛋白的生物特性，或者增强尿激酶原和t—PA(人组织型纤溶酶原激活剂)的活性。
According to reports, Nattokinase has plasmin-like bio-characteristic that lyses fibrin directly, or increases activity of pro-urokinase and t-PA (Tissue Plasminogen Activator).
据报道，纳豆激酶有和胞浆素一样的可直接分解纤维蛋白的生物特性，或者增强尿激酶原和t—PA(人组织型纤溶酶原激活剂)的活性。
According to reports, Nattokinase has plasmin-like bio-characteristic that lyses fibrin directly, or increases activity of pro-urokinase and t-PA (Tissue Plasminogen Activator).
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