• 方法应用流式细胞术细胞免疫荧光染色技

    Methods It was studied by flow cytometry and immunofluorescence.

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  • 方法应用流式细胞分析人胃癌MGC803细胞DADS处理前后细胞周期分布的变化。

    Methods cell cycle of MGC803 cell treated with DADS in vitro was analyzed by flow cytometry.

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  • 方法应用流式细胞术检测16正常人32肾癌患者外周血淋巴细胞CD25Ki67的表达。

    Methods Peripheral blood lymphocytes CD25 and Ki67 were detected by flowcytometry in 32 patients with RCC and 16 healthy individuals served as controls.

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  • 方法应用流式细胞术检测35急性淋巴细胞白血病患者骨髓细胞DNA含量(DI)、倍体细胞增殖活性(PI)。

    Methods The DNA index (DI) and proliferation index (PI) in aspirated myeloid cells were determinated in 35 patients with ALL by flow cytometry (FCM).

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  • 方法应用流式细胞测定184消化道恶性肿瘤患者癌细胞DNA含量,分析DNA胃肠道恶性肿瘤生物学行为关系。

    Methods DNA ploidy of 184 cases with malignant cancer of the gastrointestinal tract was measured by flow cytometry. The DNA ploidy and cell cycle distribution were analyzed.

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  • 方法新鲜NSCLC标本28例,应用流式细胞术测定CD44,同时测定凋亡细胞癌旁组织进行比较。

    Methods With flow cytometry, expression of CD44 and apoptosis cell peak were detected in 28 fresh specimens of NSCLC and normal pulmonary specimens.

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  • 目的应用流式细胞术建立一种准确外周血酸性细胞及其相关分子快速测定方法

    AIM: to set up an accurate and rapid method to detect the phenotype of peripheral blood eosinophils using flow cytometry.

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  • 方法应用流式细胞术检测80大肠癌标本CD34表达

    Methods: Flow cytometry (FCM) was used to detect the expression of CD34 in 80 cases cancer of colon.

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  • 目的探讨应用流式细胞术细胞荧光染料CFSE检测T淋巴细胞各亚群增殖反应方法学。

    Objective To evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using flow cytometry and carboxyfluorescein diacetate succinimidyl ester(CFSE) labeling assay.

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  • 方法应用基因芯片、反转录PCR(RT-PCR)、流式细胞术方法从分子蛋白水平检测两种细胞CXCR4表达情况。

    Methods: Gene chip and RT-PCR were used to study expression of CXCR4 mRNA in two cell lines and the CXCR4 surface expression was also examined by flow cytometry analysis.

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  • 方法应用免疫细胞化学流式细胞术方法

    Methods The immunocytochemical and flow cytometry were used.

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  • 方法应用MTT检测不同浓度5-氮-2’-脱氧胞苷胆管癌细胞QBC939存活率影响,应用流式细胞术检测5-氮-2’-脱氧胞苷QBC939细胞生长周期凋亡的影响。

    Methods The survival rate and apoptosis rate of QBC939 after being treated with different density of 5-aza-2'-deoxycytidine were detected by using the method of MTT and flow cytometry.

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  • 方法:四氮唑蓝色法(MTT法)观察梁金菇多糖HL 6 0细胞抑制率,应用形态学流式细胞术DNA凝胶电泳等方法检测细胞凋亡的发生。

    METHODS: The proliferation inhibition effects of LJPS on HL-60 cells was observed by MTT colorimetry. Apoptosis was detected by morphology, flow cytometry and DNA agarose gel electrophoresis.

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  • 方法应用形态学观察MTT分析法,DNA梯状电泳流式细胞检测,研究紫杉醇成骨肉瘤细胞SO SP- 96 0 7细胞效应

    Methods: The cytotoxic effect of paclitaxel on human osteosarcoma cell line SOSP-9607 was examined by MTT assay, morphological observation, gel electrophoresis of DNA, and cell morphology.

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  • 方法应用形态学观察MTT分析法,DNA梯状电泳流式细胞检测,研究紫杉醇成骨肉瘤细胞SO SP- 96 0 7细胞效应

    Methods: The cytotoxic effect of paclitaxel on human osteosarcoma cell line SOSP-9607 was examined by MTT assay, morphological observation, gel electrophoresis of DNA, and cell morphology.

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