方法应用流式细胞术和细胞免疫荧光染色技术。
Methods It was studied by flow cytometry and immunofluorescence.
方法应用流式细胞术分析人胃癌MGC803细胞在DADS处理前后细胞周期分布的变化。
Methods cell cycle of MGC803 cell treated with DADS in vitro was analyzed by flow cytometry.
方法应用流式细胞术检测16例正常人和32例肾癌患者外周血淋巴细胞CD25、Ki67的表达。
Methods Peripheral blood lymphocytes CD25 and Ki67 were detected by flowcytometry in 32 patients with RCC and 16 healthy individuals served as controls.
方法应用流式细胞术检测了35例急性淋巴细胞性白血病患者骨髓细胞DNA含量(DI)、倍体和细胞增殖活性(PI)。
Methods The DNA index (DI) and proliferation index (PI) in aspirated myeloid cells were determinated in 35 patients with ALL by flow cytometry (FCM).
方法应用流式细胞术测定184例消化道恶性肿瘤患者癌细胞DNA含量,并分析DNA倍体和胃肠道恶性肿瘤生物学行为关系。
Methods DNA ploidy of 184 cases with malignant cancer of the gastrointestinal tract was measured by flow cytometry. The DNA ploidy and cell cycle distribution were analyzed.
方法取新鲜NSCLC标本28例,应用流式细胞术测定CD44,同时测定凋亡细胞峰,并与癌旁肺组织进行比较。
Methods With flow cytometry, expression of CD44 and apoptosis cell peak were detected in 28 fresh specimens of NSCLC and normal pulmonary specimens.
目的:应用流式细胞术,建立一种准确、外周血嗜酸性细胞及其相关分子的快速测定方法。
AIM: to set up an accurate and rapid method to detect the phenotype of peripheral blood eosinophils using flow cytometry.
方法:应用流式细胞术检测80例大肠癌标本中CD34的表达。
Methods: Flow cytometry (FCM) was used to detect the expression of CD34 in 80 cases cancer of colon.
目的探讨应用流式细胞术和活细胞荧光染料CFSE检测T淋巴细胞各亚群增殖反应的方法学。
Objective To evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using flow cytometry and carboxyfluorescein diacetate succinimidyl ester(CFSE) labeling assay.
方法:应用基因芯片、反转录PCR(RT-PCR)、流式细胞术等方法从分子和蛋白水平检测两种细胞系中CXCR4的表达情况。
Methods: Gene chip and RT-PCR were used to study expression of CXCR4 mRNA in two cell lines and the CXCR4 surface expression was also examined by flow cytometry analysis.
方法应用免疫细胞化学和流式细胞术方法。
Methods The immunocytochemical and flow cytometry were used.
方法应用MTT法检测不同浓度的5-氮-2’-脱氧胞苷对胆管癌细胞株QBC939存活率的影响,应用流式细胞术检测5-氮-2’-脱氧胞苷对QBC939细胞生长周期及凋亡率的影响。
Methods The survival rate and apoptosis rate of QBC939 after being treated with different density of 5-aza-2'-deoxycytidine were detected by using the method of MTT and flow cytometry.
方法:四氮唑蓝比色法(MTT法)观察梁金菇多糖对HL 6 0细胞的抑制率,应用形态学、流式细胞术、DNA凝胶电泳等方法检测细胞凋亡的发生。
METHODS: The proliferation inhibition effects of LJPS on HL-60 cells was observed by MTT colorimetry. Apoptosis was detected by morphology, flow cytometry and DNA agarose gel electrophoresis.
方法:应用形态学观察,MTT分析法,DNA梯状电泳,流式细胞术检测术,研究紫杉醇对成骨肉瘤细胞系SO SP- 96 0 7的细胞毒效应。
Methods: The cytotoxic effect of paclitaxel on human osteosarcoma cell line SOSP-9607 was examined by MTT assay, morphological observation, gel electrophoresis of DNA, and cell morphology.
方法:应用形态学观察,MTT分析法,DNA梯状电泳,流式细胞术检测术,研究紫杉醇对成骨肉瘤细胞系SO SP- 96 0 7的细胞毒效应。
Methods: The cytotoxic effect of paclitaxel on human osteosarcoma cell line SOSP-9607 was examined by MTT assay, morphological observation, gel electrophoresis of DNA, and cell morphology.
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