目的探讨实时荧光定量聚合酶链反应(FQ - PCR)在检测外周血清及单核细胞中hcvRNA含量的临床应用价值。
Objective To investigate the clinical application of real time fluorescence quantitive Polymerase Chain Reaction (FQ-PCR) in detection of HCV RNA in serum and peripheral blood monocular cells (PBMC).
经实时荧光定量逆转录聚合酶链反应验证了基因芯片准确可靠。
The results of realtime RTPCR proved the accuracy of the gene chip.
方法采用实时荧光定量聚合酶链反应和免疫组化染色法检测96例食管癌患者的食管癌组织和癌旁正常组织Caspase-8表达。
Methods We quantified Caspase-8 expression levels in 96 esophageal cancer issues and their matched normal esophagus tissues by using real-time PCR assay and immunohistochemistry staining.
方法采用实时荧光定量聚合酶链反应和免疫组化染色法检测96例食管癌患者的食管癌组织和癌旁正常组织Caspase-8表达。
Methods We quantified Caspase-8 expression levels in 96 esophageal cancer issues and their matched normal esophagus tissues by using real-time PCR assay and immunohistochemistry staining.
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