采用套式聚合酶链反应方法对合并TTV感染情况进行回顾性研究。
TTV infection in the patients was retrospectively analyzed with nested PCR detection of TTV DNA in serum.
目的建立套式聚合酶链反应（PCR）加限制性酶切分析方法检测人巨细胞病毒（HCMV）。
Objectives To establish an nested (polymerase chain reaction) PCR analytic method with restrictive enzyme to detect human cytomegalovirus (HCMV) rapidly in clinics.
方法从确诊的HIV - 1感染者全血样本中提取基因组dna，经套式聚合酶链反应(PCR)扩增和测序。
Methods Genomic DNA was extracted from the whole blood samples collected from HIV-1 positive patient. Nested PCR was carried out and PCR products were purified and used directly for sequencing.
方法采用套式聚合酶链反应（nPCR）对191例、6种不同人群血清中SENVDNA进行D亚型和H亚型分析。
METHODS SENV-D and SENV-H DNA were detected in 191 cases who were divided into 6 groups with nested-PCR.
方法采用套式聚合酶链反应（nPCR）对191例、6种不同人群血清中SENVDNA进行D亚型和H亚型分析。
METHODS SENV-D and SENV-H DNA were detected in 191 cases who were divided into 6 groups with nested-PCR.
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