方法：采用序列特异性引物聚合酶链反应的方法对汉族72例uc患者和314例正常对照者HLA - DRB1基因分型。
Methods: By using polymerase chain reaction-sequence specific primers, frequency of HLADRB1 alleles in 72 patients with UC and 314 healthy controls were detected.
方法运用聚合酶链反应-序列特异性引物（PCR-SSP）法，对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
该研究通过设计合成hla基因序列特异性引物，建立了HLA -DR基因分型的套式扩增和直接扩增pcr -SSP技术，并在骨髓移植配型中进行了应用。
In this study, a nested PCR SSP and a direct amplification PCR SSP protocols for HLA DR genotyping were developed and were used in the selection of matched donor for sibling BMT.
比较基于该区段及基于基因组全序列进行的系统进化分析结果，进一步证明该区段可替代全序列用于基因分型。
Phylogenetic analysis results are compared based on the full length and the selected region to further prove the statistical results.
TTV的基因分型从已报道的TTVDNA部分基因序列来看，TTV DNA具有高度变异性，采用聚合酶链反应(PCR)检测血清ttv DNA是诊断ttv感染的主要手段。
Genotyping of TTV: according to reported part gene order of TTV DNA, it has high variability. To detect TTV DNA in blood serum by PCR is the maim means to diagnosis the TTV infection.
基因测序分型方法被认为是人类白细胞抗原分型的金标准，它可得出精确的核苷酸序列。
Gene sequencing is considered to be the golden standard for HLA typing, which can obtain exact nucleotide sequence.
根据29株NDVHN基因部分编码序列绘制NDV系统发育进化树，发现F及HN基因的分型结果大致相同。
Phylogenic tree from 29 strains were draw based on the NDV HN gene (part segment), and the result is similar to that of F gene.
然后分别提取84例拟进行造血干细胞移植病人及家系成员的外周血d NA，采用RSCA和序列特异性引物体外基因扩增(PCRssp)法平行对照对HLA A基因位点进行分型。
DNA samples of related hematopoietic stem cell transplant donor-recipients were extracted from peripheral blood cells. HLA-A loci were typed both by RSCA and PCR-SSP.
然后分别提取84例拟进行造血干细胞移植病人及家系成员的外周血d NA，采用RSCA和序列特异性引物体外基因扩增(PCRssp)法平行对照对HLA A基因位点进行分型。
DNA samples of related hematopoietic stem cell transplant donor-recipients were extracted from peripheral blood cells. HLA-A loci were typed both by RSCA and PCR-SSP.
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