目的:观察兔骨髓间充质干细胞的生长特点及其在诱导条件下的成骨能力。
OBJECTIVE To observe the growth characteristics of rabbit bone marrow-derived mesenchymal stem cells and their osteogenic potential under the induced condition.
方法体外培养获取生长状态良好的兔骨髓间充质干细胞,进行成软骨诱导,观察并检测其生物学特性。
Methods Rabbit BMSCs were cultured in vitro induced to chondrocytes, and then the biologic characters of the chondrocyte were observed and tested.
目的:拟建立一套体外分离培养及大量扩增兔骨髓间充质干细胞的方法,并对细胞生物学特性进行观察。
OBJECTIVE: To establish a method for in vitro isolation and cultivation of BMSCs from rabbits and to study their biological characteristics.
目的:体外培养兔骨髓间充质干细胞并定向诱导分化为软骨细胞,探讨体外诱导分化为软骨的方法和条件。
OBJECTIVE: to build a method of differentiation of rabbit BMSCs into chondrocytes in vitro and to determine the factors involved in the differentiation process.
方法体外培养获取生长状态良好的兔骨髓间充质干细胞,进行成骨诱导,观察并检测成骨细胞的生物学特性。
Methods Rabbit BMSCs were cultured in vitro induced to osteoblasts, and then the biologic characters of the BMSCs was observed and tested.
材料:5月龄新西兰大白耳兔用于骨髓间充质干细胞的分离培养。
MATERIALS: New Zealand big-eared rabbits aged 5 months were used for isolation and culture of BMSCs.
老龄兔;骨髓间充质干细胞;细胞移植;心肌梗死。
Aged rabbits; Mesenchymal stem cells (MSCs); Myocardial infarction; cell transplantation.
目的探讨自体骨髓间充质干细胞对促进兔前交叉韧带重建后腱骨愈合的影响。
Objective to study the effect of autologous bone mesenchymal stem cell (bMSC) transplantation on tendon-bone healing after anterior cruciate ligament (ACL) reconstruction.
目的:观察透明质酸钠混合溶液复合骨髓间充质干细胞修复兔退变椎间盘的效果。
OBJECTIVE: To observe the effect of bone mesenchymal stem cells (BMSCs) mixed with sodium hyaluronate solution in repair of disc degeneration in rabbits.
方法:取健康新西兰幼兔骨髓,密度梯度离心法分离骨髓间充质干细胞并进行体外扩增培养、成骨诱导分化及鉴定。
METHODS: Bone marrow was obtained from healthy New Zealand rabbits, by using density gradient centrifugation BMSCs were isolated and were then cultured in vitro.
实验以藻酸盐凝胶为支架复合骨髓间充质干细胞,观察修复兔腰椎间盘退行性变的能力。
This study observed the effect of bone marrow mesenchymal stem cells (BMSCs) co-cultured with alginate gel on repairing the degeneration of lumbar intervertebral disc.
实验以藻酸盐凝胶为支架复合骨髓间充质干细胞,观察修复兔腰椎间盘退行性变的能力。
This study observed the effect of bone marrow mesenchymal stem cells (BMSCs) co-cultured with alginate gel on repairing the degeneration of lumbar intervertebral disc.
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