目的：对广西中药三七主要有效成分三萜皂甙合成关键酶法昵基焦磷酸合酶的基因进行体外扩增、克隆及序列分析。
Object: A cDNA encoding farnesyl pyrophasphate synthase (FPS) that is one of key enzymes in the synthesis of triterpene saponins from Panax notoginseng (Burkill) F. H. Chen ex C. Y. Wu & K. M.
通过序列同源比对及系统发育进化分析，发现克隆到的序列与其它物种DOB基因具有很高的同源性。
Sequence of SLA-DOB1 was cloned that was high homology with the other species by multiple sequences alignment and phylogeny evolution analysis.
方法采用基因克隆、测序及生物信息学技术，分析HLA新等位基因与HLA已知基因序列的差异。
Methods Molecular cloning, sequencing and bioinformatics techniques were used to identify the difference between the new HLA allele and other known alleles.
方法：采用PCR，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。
Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.
方法：采用PCR，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。
Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.
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