观察原代和传代细胞的生长情况。
The primary and passages of retinal progenitors were observed.
使用标准培养液的传代细胞均为阴性。
The cells, which were cultured in standard culture medium, were negative.
最后，气管抽吸的原代细胞和之后的传代细胞有同样模式。
Finally, primary cells from tracheal aspirates behaved in an identical manner as later passage cells.
目的探索CD 40反义rna对肿瘤传代细胞K562细胞株的影响。
Objective To explore the effects of CD40 anti sense RNA on K562 cell line.
参考品病毒滴定采用金黄色地鼠传代细胞(bhk—2 1)蚀斑滴定法。
早期传代细胞染色体数出现非整倍体和非稳定性畸变，同时有两个标记染色体；
There were aneuploid and instable structure changes in chromosomes, and two marker chromosomes at the same time.
流感病毒在狗肾传代细胞(MDCK)生长良好，而在其变异株mdck - L细胞生长受阻。
Influenza virus growth well in MDCK cell, but a variant of MDCK cell line (MDCKL cells) greatly restricted the growth of influenza viruses.
结论：所建立的RT _PCR方法可自病毒感染的小鼠脑组织和传代细胞中检测WEE病毒的基因组rna。
Conclusions: a specific RT_PCR assay was established for detecting the genomic RNA of WEE virus from infected Suckling mice and culture cells.
方法收集非典型肺炎患者的各种临床标本，用狗肾传代细胞(MDCK)进行病原体分离，并运用血清学和分子生物学方法进行鉴定。
Methods Pathogens were isolated from variety of samples collected from atypical pneumonia patient by using MDCK cells, and identified with serological and molecular methods.
从临床发病犬采集粪便样品，以F81传代细胞进行病毒分离，经血球凝集实验(HA)和血球凝集抑制实验(HI)初步鉴定为犬细小病毒。
The HL-01 isolate of canine parvovirus was propagated on F81 cell. And identified by the haem agglutination test (ha) and haem agglutination inhibition test (hi).
即使在连续传至128代的CSFV39 - PK 15传代细胞中，CSFV仍持续存在：呈免疫荧光抗体反应阳性和RT - PCR检测阳性。
Even in the 128th CSFV39-PK15 passage cells, we still could detect the existence of CSFV39. Both immunofluorescence antibody test and RT-PCR assay showed positive results.
从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
细胞传代20次后，仍生长良好。
目的探讨血管内皮细胞在传代过程中的衰老过程及机制。
Objective To study the process of aging and its potential mechanisms during passage of vascular endothelial cells.
结果：原代培养的RPE细胞含大量色素呈多角形，传代的细胞透明呈梭形。
Results: Primary cultured RPE cells contained large amount of pigments in polygonal shape. Passages of cultured RPE cells presented transparent spindle shape.
本文就胚胎质量和胚胎类型分化抑制物培养基传代时间和消化液等方面对胚胎干细胞分离克隆的影响进行了讨论。
Influences of embryo quality and type differentiation stayer media passage and digestion fluid on embryonic cell separation and cloning were discussed respectively.
方法传代得到单个HERG-HEK细胞，应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线（激活、失活、复活和去活化）。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法分别自3周龄大耳白兔关节软骨和半月板分离软骨细胞，行单层传代培养和离心管培养。
Methods Chondrocytes isolated from articular cartilage and meniscus of rabbits aged 3 weeks were respectively passaged in monolayer and cultured in centrifuge tube.
方法用狗肾传代(MDCK)细胞和鸡胚双腔法进行流感病毒分离。
Methods The influenza virus isolation was performed by MDCK cells and embryonated eggs.
方法：用胰酶消化法培养兔rpe细胞并传代。
Methods: The rabbit RPE cells were cultured with trypsin enzyme-digesting technique and passaged in vitro.
方法将人肝门部胆管癌标本进行细胞培养，传代。
Methods The fresh human hilar cholangiocarcinoma specimen was cultured.
小鼠骨髓内皮细胞经过长期培养后，已转化为内皮细胞株，已传代20次，其增殖能力没有减退。
Murine bone marrow endothelial cell line could be established after long time culture and there is no significant reduction in their proliferation potency after 20 passages.
目的探索人心房肌细胞的原代及传代培养方法。
Objective To explore a method for primary culture and subculture of human atrial myocardial cells.
结论：利用多次差速传代可从混合培养的原代细胞中获得纯化的牙囊细胞。
Conclusion: Dental follicle cells can be purified by several differential passages from the mixed primarily cultured cells.
经基因工程化及传代后，仍具干细胞特性，可作为基础研究及神经移植供体，具有广阔应用前景。
Since they retain their characteristics and differentiation ability after passages and genetic engineering, they can be applied widely in basic research and as grafts in neural transplantation.
方法：原位传代培养羊水细胞并制备染色体，G显带分析核型，产后随访。
Methods:Amniotic fluid cells were cultured in situ and their karyotypes were analyzed by G band, followed them after delivery.
采用无血清液体培养基、DMEM培养基和MEM培养基进行PK15细胞的培养，观察细胞增殖及传代效果，确定无血清液体培养基的营养功能。
PK15 cells were cultured with free-blood serum media, DMEM media and MEM media in other to observe the effect of cell proliferation, cell transfer and define the ability of nutrition.
方法：采用细胞培养的方法建立稳定传代的胰腺癌hs 766t细胞系，体外培养人脐带静脉内皮细胞。
Methods: We built the pancreatic cancer cell group HS 766t and human endothelium cell in the umbilical vein by the way of cell culture in vitro.
另外研究血管内皮生长因子(VEGF)和传代培养对细胞分化、扩增动力学和细胞凋亡的影响。
In addition, we tested the effect of vessel endothelial growth factor (VEGF) and passage on cell differentiation, expansion kinetics and apoptosis.
自成年新西兰兔股骨转子部取得骨髓基质细胞进行传代培养，所得骨髓基质细胞再与钙磷陶瓷载体复合培养制成复合人工骨。
Bone marrow cells from New Zealand white rabbits were cultured to obtain stromal cells, which were then cultured with the carriers to make the artificial composite bone graft.
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