The replication ability of mutation strains is weaker than wild strains, and YVDD strains have stronger replication ability than YIDD strains.
突变株的复制能力低于野生株，YVDD突变株复制能力强于YIDD突变株。
Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.
结果经pcr方法、酶切鉴定及直接测序鉴定出YMDD、YVDD和YIDD质粒构建成功，且该方法具有较好的敏感性和特异性。
Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.
结果经pcr方法、酶切鉴定及直接测序鉴定出YMDD、YVDD和YIDD质粒构建成功，且该方法具有较好的敏感性和特异性。
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