Results The cloned antibody variable regions genes were approved functional by sequencing.
结果序列测定初步确定所克隆的是功能性抗体可变区基因。
Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.
方法利用RT -PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。
Methods The genes encoding antibody variable regions were cloned by RT PCR from hybridoma cells and expressed on bacterial phage surface.
方法利用RTPCR 方法扩增抗体可变区基因并表达于噬菌体表面。
Methods The genes encoding antibody variable regions were cloned by RT PCR from hybridoma cells and expressed on bacterial phage surface.
方法利用RTPCR 方法扩增抗体可变区基因并表达于噬菌体表面。
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