Methods kidney cortex explanted into the serum-free culture medium gave rise to a primary culture of kidney epithelial cells. After confluence, cells were subcultured using Trypsin-EDTA digestion.
方法取肾皮质组织块，应用无血清培养液进行原代培养，融合后用胰蛋白酶- EDTA消化传代。
The fibroblasts grew along with the growth of eccrine sweat gland cells, and they were separated by digesting with 0 % trypsin and 0 0% ethylenediaminetetraacetic acid (EDTA).
用质量分数为0 。 %的胰酶和0 .0 %的乙二胺四乙酸 （EDTA）消化分离汗腺细胞和成纤维细胞 ；
The fibroblasts grew along with the growth of eccrine sweat gland cells, and they were separated by digesting with 0 % trypsin and 0 0% ethylenediaminetetraacetic acid (EDTA).
用质量分数为0 。 %的胰酶和0 .0 %的乙二胺四乙酸 （EDTA）消化分离汗腺细胞和成纤维细胞 ；
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