Albumin, serum in the culture medium decreased the transfection efficiency.
培养液中的白蛋白、血清可降低转染效率。
The transfection efficiency decrease with the increase of the alkyl chains.
随着碳链长度的增加，表面活性剂的转染效率随之降低；
However, low genetic transfection efficiency limits their clinical application.
然而低转染效率限制了其在临床上的应用。
Additionally, PEI is considered to exhibit high transfection efficiency and endosomal disrupting capacity.
此外，PEI也被认为具有高转染率和使内涵体破裂的能力。
In the second part, we investigated the influence of gene delivery vectors on gene transfection efficiency.
其二，研究了基因载体的结构对于基因转染效率的影响。
The original chitosan carrier is modified to improve the transfection efficiency and the targeted property to respiratory cells.
对原始壳聚糖载体进行改性，提高其转染效率和对于呼吸道细胞的靶向性。
If the transfection efficiency is improved, gold nanoparticles will be very useful as mediator for gene transfection in living cells.
如果能够提高基因的转染率，那么在对活细胞进行基因转染时，纳米金就可以作为一种费用有用的媒介。
Conclusion: transfection of gastric carcinoma with HSV-TK has higher transfection efficiency. MKN28TK cells are significantly sensitive to GCV.
结论：逆转录病毒介导的HSV -TK系统对人胃癌细胞有较高的转染效率，GCV对转染阳性的胃癌细胞有显著的杀伤作用。
The invention aims at solving the technical problem of proving a cationic liposome with high transfection efficiency and tumor vascular targeting.
本发明所要解决的技术问题是提供一种转染效率高且具有肿瘤血管靶向性的阳离子脂质体。
We choose Non small cell lung adenocarcinoma cell line H1299 as cell model for its low endogenic SEPT6 expression and high transfection efficiency.
我们选取了转染效率较高且SEPT 6基础表达量不是很高的非小细胞肺腺癌h 1299作为细胞模型。
Adenovirus vectors have the characteristics of high transfection efficiency and low cytotoxicity, and they have become most widely used vectors for gene therapy.
摘要腺病毒载体具有高效转染和低细胞毒性的特点，已成为基因治疗研究中应用最广泛的载体之一。
Objective to prepare chitosan nanoparticles as gene vectors so as to investigate their structural characteristics and cell-gene transfection efficiency in vitro.
目的制备基因载体壳聚糖纳米粒，研究其结构特征及其体外对细胞的转染活性。
The formulated complexes exhibited a considerably higher transfection efficiency against FOL receptor over-expressing KB cells than FOL receptor deficient A549 cells.
与缺乏FOL受体的A549细胞相比，由此而来的合成物在过量表达FOL受体的KB细胞中有更高的转染率。
ConclusionUsing GFP as a report gene in eukaryotic cell transfection, the lipofectamine method could get a higher transfection efficiency and higher survival rate for cells.
结论采用绿色荧光蛋白为报告基因的真核细胞转染技术中，脂质体法是效率高、安全性大的方法。
We developed new methods to pattern cells on both hydrophilic mica surface and hydrophobic PDMS surface and investigated the gene transfection efficiency on the patterned cells.
分别研究了在亲水性云母表面和疏水性PD MS表面细胞图案化的方法，并且对这些图案化细胞的基因转染效率进行了研究。
The previous research work of our lab has demonstrated that the arginine modified chitosan and hexadecylene modified chitosan could improve the gene transfection efficiency obviously.
我们实验室的前期工作显示，采用精氨酸和十六烷基修饰壳聚糖，可以明显改善其转基因效率。
AS-ODN/DOTAP complex could exhibited more higher transfection efficiency than free AS-ODN and the transfection efficiency was also influenced by many other factors such as cell density etc.
DOTAP复合物的转染效率明显高于游离AS-ODN；细胞是否贴壁生长及贴壁生长的细胞密度也影响AS-ODN的转染效率。
Objective To construct the retroviral(RV) vector with report gene enhanced green fluorescent protein(EGFP) and to explore the gene transfection efficiency of RV on SK-N-SH neuroblastoma cells.
目的构建携带报告基因增强型绿色荧光蛋白（EGFP）的反转录病毒载体，并且探讨病毒载体对SK-N-SH神经母细胞瘤细胞株的感染效率。
Due to the specific bioactivity of peptides, peptide-based gene and drug delivery systems could achieve improved gene transfection efficiency, enhanced drug targeting property and reduced side-effect.
将具有特殊生理功能的多肽作为基因或药物载体、或用于药物修饰等，可以提高基因转染效率，增强药物的靶向治疗效果，降低药物的毒副作用。
Objective To study the expression efficiency of CYP2J3 gene in rat smooth muscle cells (SMCs) by liposome mediated and naked plasmid transfection.
目的比较脂质体介导法和裸质粒直接转染法导入CYP2J3基因表达的效率。
The efficiency of transfection is examined by Flow CytoMeter and the cytotoxicity is evaluated with MTTassay.
流式细胞仪检测转染效率，MTT法检测细胞毒性。
Objective To explore the efficiency of transfection of antisense oligonucleotides HA-2741 by ultrasound mediated liposome microbubbles.
目的探讨超声介导脂质体微气泡转染反义寡核苷酸ha- 2741的有效性。
The major drawback of being a gene delivery vector is the low efficiency of transfection, so the scientists have been trying to increase the efficiency of chitosan with the methods of modifications.
壳聚糖作为基因载体的主要缺陷是转基因效率比较低，所以研究者们试图通过修饰以增加壳聚糖的转基因效率。
The major drawback of being a gene delivery vector is the low efficiency of transfection, so the scientists have been trying to increase the efficiency of chitosan with the methods of modifications.
壳聚糖作为基因载体的主要缺陷是转基因效率比较低，所以研究者们试图通过修饰以增加壳聚糖的转基因效率。
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