Differentially expressed genes were identified by real-time quantitative PCR.
采用实时荧光定量PCR鉴定高表达基因。
Results The sensitivity of the established real time quantitative PCR was at 10 -4 level.
结果建立的实时荧光定量PCR方法的灵敏度为10-4水平。
Real time quantitative PCR has been widely used in various areas of plant pathology research.
实时荧光定量pcr广泛应用于植物病理学研究的各个领域。
Cytology on BKV infection was carried out and was compared with PCR. Cytology has less sensitivity and specificity than real-time quantitative PCR.
开展了BK病毒感染细胞学研究，并与PCR方法作了比较，认为细胞学检查灵敏度和特异度均低于定量pcr法。
The significance, content and method of short time quantitative precipitation forecasting and its recent advances worldwide have been systematically introduced.
系统地介绍了短期定量降水预报研究的意义、内容及方法，综述了近年来国内外的研究进展。
Methods The expression of TUSC3 gene in 20 cases of pancreatic cancer and 6 cases of normal pancreas were detected by oligonucleotide microarray and real-time quantitative PCR.
方法利用寡核苷酸基因芯片技术和实时定量pcr技术检测20例胰腺癌组织和6例正常胰腺组织中TUSC3基因的表达。
DNA methodology and gestational age had the largest effects on test performance, with real-time quantitative polymerase chain reaction (RTQ-PCR) outperforming conventional PCR.
所用的DNA方法学和所处的妊娠期对检测精度有着最大的影响，实时定量多聚酶链反应(RTQ - PCR)的检测精度超过常规的PCR。
At present, the com-mon methods include multiplex PCR, Real Time Quantitative PCR, in situ PCR and immuno-polymerase chain reaction. They have different principles, advantages and disadvantages.
目前常用的PCR法有多重PCR技术、实时荧光定量PCR技术、原位PCR技术及免疫PCR技术，各种技术有不同的原理及优缺点。
At present, the com-mon methods include multiplex PCR, Real Time Quantitative PCR, in situ PCR and immuno-polymerase chain reaction. They have different principles, advantages and disadvantages.
目前常用的PCR法有多重PCR技术、实时荧光定量PCR技术、原位PCR技术及免疫PCR技术，各种技术有不同的原理及优缺点。
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