Sucralose was purified by column chromatograph and determined by thin layer chromatograph.
采用薄层色谱法定性，以柱色谱法分离提纯三氯蔗糖。
The extracts were separated by silica gel column chromatography, thin-layer chromatography and paper chromatography.
提取液经柱层析分离及薄层层析和纸层析进一步纯化。
Methods: to isolate the alkaloids, the solvent-extraction together with the column, chromatography and thin-layer chromatography methods were used.
方法：采用了溶媒分离法结合柱层析法、薄层层析法进行成分分离。
Pure TS99 could be obtained from pretreatment fermentation broth through macroreticular adsorbent, silica gel column chromatography and thin-layer chromatography.
发酵液经过预处理后，依次用大孔树脂吸附，硅胶柱吸附层析和薄层层析等分离方法即可获得农用抗生素TS99纯品。
Pure TS99 could be obtained from pretreatment fermentation broth through macroreticular adsorbent, silica gel column chromatography and thin-layer chromatography.
发酵液经过预处理后，依次用大孔树脂吸附，硅胶柱吸附层析和薄层层析等分离方法即可获得农用抗生素TS99纯品。
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