Objective to clone the gene activating transcription factor 5 (ATF5), construct the expression plasmid and detect the localization of ATF5 in cultivated cells.
目的克隆激活转录因子(atf)5，构建其真核表达载体，观察其在细胞中的表达定位。
AIM: to construct plasmid vector of RNA interference specific for matrix metalloproteinases (MMP) -9 and observe the silencing effect on gene expression of MMP-9 in macrophage of mice.
目的：构建基质金属蛋白酶9靶向的RNA干扰质粒载体，观察其对小鼠巨噬细胞基质金属蛋白酶9基因表达的沉默作用。
We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.
我们使用了依赖HCVIRES表达萤火虫荧光素酶的报告基因质粒。
Introduction The construction and transfection of eukaryotic expression plasmid are commonly molecular biology method used in medical research.
序言真核表达质粒的构建和转染是目前医学研究中常用的分子生物学手段。
Objective To study the expression efficiency of CYP2J3 gene in rat smooth muscle cells (SMCs) by liposome mediated and naked plasmid transfection.
目的比较脂质体介导法和裸质粒直接转染法导入CYP2J3基因表达的效率。
Conclusion The recombinant expression plasmid constructed by restriction enzyme cleave identification can highly express recombinant human ZP3 protein.
结论：经酶切鉴定构建的人透明带蛋白3重组表达载体可高效表达重组人zp3蛋白。
Conlusion The human FHIT recombinant eukaryotic expression plasmid was constructed successfully.
结论成功构建了重组人FHIT真核表达质粒。
Methods:One of the three internal translation initiation region(TIR) was chemically synthesized and cloned into the report plasmid, and the expression of the report gene was studied.
方法：将基因内翻译起始序列合成后克隆到上游含有起始密码和无起始密码的报告质粒中，研究表达水平的差异。
Supercoiling is necessary for the expression of recombinant plasmid DNA at transcription level.
超螺旋化对于质粒dna在转录水平上的表达是必需的。
Objective To construct the eukaryotic coexpression plasmid encoding carcinoembryonic antigen(CEA) and heat shock proteins 70 (HSP70), then detect the expression of the plasmid in vitro.
目的构建癌胚抗原（CEA）与热休克蛋白70（HSP70）的真核双表达质粒，并检测其在体外的表达。
We established the techniques of screening the plasmid expression library and cloning the immunogen genes.
建立了筛选质粒表达文库和克隆免疫原基因的技术方法。
Objective To construct the prokaryotic expression plasmid expressing woodchuck hepatitis virus core antigen (WHcAg) and prepare polyclonal antibodies.
目的构建土拨鼠肝炎病毒核心蛋白质粒并进行原核表达、抗体制备。
The sequence of the cloned SCF is same as the published report. The construction of expression plasmid is identified in a correct way.
序列分析表明所克隆的SCF序列与文献报道的一致，构建的表达载体经鉴定正确。
RESULTS: DDR2/EGFP fusion plasmid was successfully constructed. Further analysis also demonstrated that the fusion protein has similar expression and activation pattern with wild type ones.
结果：成功构建了DDR2/EGFP融合表达载体，进一步的分析也证明此融合表达载体能在细胞中正确表达并可以被配体所激活。
To clone mouse interleukin 4 (mIL-4) truncated gene, construct its eukaryotic expression plasmid pFB-mIL4 and express the truncated protein (murine IL-4 receptor antagonist).
构建小鼠il -4截短型基因真核表达质粒，表达小鼠il - 4受体拮抗体蛋白。
Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid.
目的克隆人血管紧张素转换酶2基因（ACE2），并构建其真核表达载体。
Yeast expression systems are based on the discover of plasmid in Saccharomyces cere- visiae and success of yeast transformation.
酵母表达系统是在酿酒酵母质粒的发现和酵母转化技术的成熟基础上建立起来的真核生物表达系统。
CONCLUSION: a recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed, and can be expressed stably in the NIH3T3 cells.
结论：成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒，并且该基因在NIH3T3细胞中获得了稳定表达。
Therapeutic DNA molecules and gene immunity are a new research field. The technology for aerosolized DNA plasmid and transformation and expression after inhalation are of great interest.
基因治疗和基因免疫也是近年刚兴起的一个研究领域，质粒DNA的气溶胶化技术和呼吸道吸入后的转染及表达研究，是呼吸道粘膜免疫和疾病治疗的重点。
CONCLUSION: Foreign plasmid DNA, administered via the gastrointestinal tract, may widely modulate the expression of many genes in the intestine in mice.
结论：外源质粒dna通过胃肠道途径可广泛调控肠道多种基因表达。
After the reading frame was confirmed correct by sequencing, the recombinant plasmid was transformed into expression host e.
测序表明读码框架正确后，将重组子分别转入表达宿主菌e。
After mediated by eukaryotic expression plasmid-liposome, the fused protein was introduced into C2C12 cell system.
然后经真核表达质粒-脂质体介导，导入C2C 12细胞系。
The customers should provide the gene expression plasmid which has been done the fluorescence labelling. We will provide the detail reports and microscopical photos.
技术服务完成后向客户提供详细的实验报告和荧光共聚焦显微镜照片。
The adenoviruses and the eukaryotic expression plasmid have unique practicality in preparing tumor treating medicines and radiotherapy hypersensitivity.
该腺病毒和真核表达质粒在肿瘤治疗药物、放射治疗增敏的制备中具有独特的实用价值。
The expression condition of plasmid pET-tmcC has been explored, but at all the testing temperature, IPTG concentration and different hosts protein expression was not found.
将重组质粒转化至大肠杆菌宿主中，对目的蛋白进行诱导表达，并对诱导表达条件如温度、IPTG浓度等进行了优化。
The discussion was mainly focused on the application of these detection methods, the problem result in the virus extraction progress, and the construction of expression plasmid having LSV CP gene.
重点讨论了各种检测方法在实际中的应用，百合无症病毒提取过程中需要解决的问题，外壳蛋白基因在大肠杆菌中表达载体的构建。
In this dissertation, a partial fragment of NAD-SDH from apple fruit was cloned, the NAD-SDH antisense expression plasmid was constructed, and the antisense expression has been doing;
本研究克隆了苹果NAD-SDH 基因的部分cDNA 序列，构建了其反义表达载体，并正在通过农杆菌浸染法将该基因反义转化至蔷薇科植物中。
In this dissertation, a partial fragment of NAD-SDH from apple fruit was cloned, the NAD-SDH antisense expression plasmid was constructed, and the antisense expression has been doing;
本研究克隆了苹果NAD-SDH 基因的部分cDNA 序列，构建了其反义表达载体，并正在通过农杆菌浸染法将该基因反义转化至蔷薇科植物中。
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